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. Author manuscript; available in PMC: 2007 Nov 1.
Published in final edited form as: Protein Expr Purif. 2006 Dec 12;53(1):153–163. doi: 10.1016/j.pep.2006.12.003

Fig. 2.

Fig. 2

Western blot analysis of the CB2-fusion proteins (107, 122, 125, 127, 129, 130, and 131). The recombinant receptor was expressed in E. coli BL21(DE3) cells upon induction with 0.5 mM IPTG at 20 °C for 40 h. Cells were collected by centrifugation and cell membranes obtained as described in Materials and methods. After separation on 10% SDS-PAGE, proteins were electroblotted onto nitrocellulose membranes and probed with either anti-His tag HisProbe reagent or with antibodies against CB2 or MBP.