Fig. 5.
Cleavage of CB2-125 fusion with TEV protease. The reaction was performed by mixing pre-purified (Ni-NTA chromatography) micellar solutions of CB2-125 fusion with Tev protease at a molar ratio of 5:1 in buffer C supplemented with indicated amounts of imidazole. The CB2-fusion and cleaved CB2 are indicated by arrows.