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. 2007 Jul 3;104(28):11568–11573. doi: 10.1073/pnas.0705054104

Fig. 1.

Fig. 1.

Characterization of DosS by EPR and UV-visible spectroscopy. (A) UV-visible characterization of DosS (3 μM in 20 mM Tris, pH 7.5) as purified, exposed to DTH or treated with DTH and reexposed to air. (Inset) Enlarged image of the 500- to 600-nm region. (B) EPR spectroscopy analysis of air-exposed DosS (6 μM in 20 mM Tris, pH 7.5). (C) UV-visible spectrum of DosS as purified, exposed to air after DTH treatment, the former treated with KCN or Fe(CN)63−. Note the characteristic met-CN peak at 540 nm. Numbers in parentheses indicate absorption maximum in nanometers.