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. 2007 Jul 3;104(28):11568–11573. doi: 10.1073/pnas.0705054104

Fig. 4.

Fig. 4.

O2, NO, and CO are regulatory ligands of DosS. (A) Effect of O2 and NO on DosS autokinase activity. Purified DosS (Fe3+) was exposed to DTH to generate the Fe2+ form and analyzed in the autokinase assay. Note the significant difference in autokinase activity between met and ferrous DosS. DosS (Fe3+) or DTH-reduced DosS (Fe2+) were exposed to proline NONOate and are indicated by the Fe3++NO and Fe2+−NO labels, respectively. (B) Effect of the heme-iron redox state on DosS autokinase activity. DosS was exposed to DTH (Fe2+) followed by treatment with Fe(CN)63− to generate met DosS. (C) Met DosS was exposed to DTH to generate the ferrous form. Both forms were exposed to CO and are indicated by the Fe3++CO and Fe2+−CO labels, respectively. Numbers indicate minutes of incubation.