Skip to main content
. 2007;39(4):736–751. doi: 10.1016/j.biocel.2006.11.006

Fig. 1.

Fig. 1

Plasmid pRJ-B used for deriving the constructs encoding Agt DnaJ chimeric proteins. (A) A plasmid map of pRJ-B: a BstBI restriction site was engineered into the pRJ30 plasmid between the coding regions for the Agt DnaJ J-domain and GF region by silent mutation of the codon for Phe74 to produce the pRJ-B plasmid. The positions of the BamHI and BstBI restriction endonuclease sites used for swapping the J-domain coding regions in the creation of the Agt DnaJ chimeric proteins are indicated. (B) Codon optimised Pfj1 J-domain coding sequence. The translated J-domain protein sequence is highlighted and the BamHI and BstBI restriction endonuclease sites used for insertion of the coding region for the J-domain into the pRJ-B plasmid are underlined.