Table 1.

Functional analysis and expression levels of mutant J-domain chimera proteins

^aMutations were assigned according to the consensus sequence detailed in Fig. 2A.

^bTo assess the effect of the mutations on functionality of the J-domains, E. coli OD259 cells producing the mutant chimera proteins were analysed for their ability to grow under non-stress and stress temperatures: ‘+’—reversal of thermosensitivity (growth at 40 °C and at 42 °C); ‘−’—no reversal of thermosensitivity (no growth at 40 °C or 42 °C); or ‘±’—partial reversal of thermosensitivity (growth at 40 °C and less or no growth at 42 °C).

^cExpression levels of the mutant proteins in E. coli OD259 were assessed by Western analysis: “+++’—higher levels than those observed for the positive control (Agt DnaJ); ‘++’—medium levels similar to those observed for Agt DnaJ; ‘+’—lower levels than those observed for Agt DnaJ.