Table 3.
Summary of qRT-PCR amplification of cartilage-selective genes in fetal cartilage and seven non-cartilage tissues.
| CV | Ct | ||||
| Interval | Symbol | Neg | NC | C | Fold |
| U133A | |||||
| 0–10% | AGC1 | 2 | 33 | 23 | 371 |
| 10–20% | NOS2A1 | 3 | 34 | 23 | 1158 |
| 20–30% | DSPG3 | 6 | 35 | 25 | 661 |
| 30–40% | BDKRB1 | 2 | 34 | 28 | 49 |
| 40–50% | MMP3 | 3 | 33 | 20 | 4988 |
| U133B | |||||
| 10–20% | C10orf49 | 7 | 35 | 23 | Unique |
| 20–30% | KIAA1718 | 30 | 27 | 8 | |
| 30–40% | LOC20118 | 4 | 34 | 29 | 20 |
| 40–50% | ASAM | 31 | 26 | 29 | |
| U133 2.0 | |||||
| 10–20% | KIAA0701 | 30 | 27 | 5 | |
| 20–30% | MGC17337 | 30 | 27 | 5 | |
| 30–40% | OSMR | 28 | 27 | 3 | |
| 40–50% | HIG2 | 30 | 25 | 21 | |
Representative genes are listed from each validation platform in order of 10% CV interval. (Neg) Number of non-cartilage tissues in which amplification was not detected. Average Ct values for each gene were calculated for both cartilage (C) and non-cartilage (NC) tissues. Where no amplification was observed the maximum Ct value (i.e. 35) was used for calculations. Fold difference (Fold) is calculated from the difference in cartilage and non-cartilage Ct values.