RT-PCR analysis of MyoD1 and α/γAChR subunit gene transcription in normal muscles and normal tissues. Only a few transcripts of MyoD1 and even less of αAChR were found in all normal muscles. In other normal tissues, MyoD1 transcripts were detected in heart (Lane 9), liver (Lane 12), and prostate (Lane 16). By contrast, transcripts of the γAChR were totally absent from other normal tissues, whereas strong transcription of αAChR could be detected in all normal muscles and very little αAChR mRNA was detected in all normal tissues.