Skip to main content
. 2000 May;2(2):92–96. doi: 10.1016/S1525-1578(10)60622-8

Figure 1.

Figure 1.

Immunoglobulin heavy chain (IgH) PCR in a monoclonal B cell process (malignant lymphoma). Ten microliters of PCR product were loaded in every lane. Serial dilutions were performed using 50 ng of template DNA as the starting quantity per reaction. Lane 1: polyclonal DNA control showing a smear pattern. Lane 2: undiluted sample at 50 ng/reaction showing a monoclonal band (∼100 bp). Lane 3: 1:10 dilution (5 ng/reaction). Lane 4: 1:100 dilution (0.5 ng). Lane 5: 1:500 dilution (0.25 ng). Lane 6: 1: 1000 dilution (0.05 ng). Lane 7: 1:1500 (0.033 ng). The monoclonal band (∼100 bp) is seen to persist at a dilution of 1:1000.