Table 2.
Precision of the TaqMan HCV Test
Variable | Input IU/ml | ||
---|---|---|---|
4000 | 80,000 | 1,600,000 | |
Total number of replicates | 60 | 60 | 60 |
Mean calculated RNA conc. (IU/mL) | 3,101 | 98,199 | 3,010,026 |
Minimum value | 1,325 | 46,611 | 1,127,390 |
Maximum value | 5,600 | 155,064 | 4,644,024 |
Within-run | |||
Standard deviation | 926 | 18,087 | 511,429 |
Coefficient of variation | 26.6% | 18.4% | 17.0% |
Total | |||
Standard deviation | 941 | 22,928 | 649,456 |
Coefficient of variation | 30.4% | 23.4% | 21.6% |
These calculations was performed as described in the National Committee for Clinical Laboratory Standards guideline EP5-T2 for within-run and total precision. Total precision includes between-run (instrument) variation and between-day variation.