Table 2.
Precision of the TaqMan HCV Test
| Variable | Input IU/ml | ||
|---|---|---|---|
| 4000 | 80,000 | 1,600,000 | |
| Total number of replicates | 60 | 60 | 60 |
| Mean calculated RNA conc. (IU/mL) | 3,101 | 98,199 | 3,010,026 |
| Minimum value | 1,325 | 46,611 | 1,127,390 |
| Maximum value | 5,600 | 155,064 | 4,644,024 |
| Within-run | |||
| Standard deviation | 926 | 18,087 | 511,429 |
| Coefficient of variation | 26.6% | 18.4% | 17.0% |
| Total | |||
| Standard deviation | 941 | 22,928 | 649,456 |
| Coefficient of variation | 30.4% | 23.4% | 21.6% |
These calculations was performed as described in the National Committee for Clinical Laboratory Standards guideline EP5-T2 for within-run and total precision. Total precision includes between-run (instrument) variation and between-day variation.