Table 2.

Primers for TCRγGR

Primer	Jγ-labeled PCR	Vγ-labeled PCR	Sequence 5′-3′	Reference
Vγ2*	NED	ACTCCAGGGTTGTGTTGGGAATCA	27, 28
Vγ3	NED	CCGCAAGGGATGTGTTGGAATCA	27, 28
Vγ9	JOE	ACGGCACTGTCAGAAAGGAATC	27, 28
Vγ10	ROX	AATCCGCAGCTCGACGCAGCA	26, 29
Vγ11	FAM	GGCTCAAGATTGCTCAGGTGG	29
Jγ1/Jγ2	NED	TAC CTG TGA CAA CAA GTG TTG TTC	5
JγP	FAM	AAG CTT TGT TCC GGG ACC AAA TAC	5
JγP1/JγP2	JOE	GAA GTT ACT ATG AGC T/CTA GTC CCT T	5

FAM, JOE and NED are fluorochromes attached to the 5′ end of the Jγ primers which were established in a previous DGGE protocol. 5 The Vγ primers were newly designed for this assay from published TCRγ variable gene sequences.

, Vγ2 also has sequence homology with gene segments Vγ4-8.