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Measurement of HPV 16 and 18 E7 in cervical cancer cell lines by TaqMan. The specificity of E7 primers for their respective HPV types were tested using RNA extracted from both Caski (HPV 16) and HeLa (HPV 18) cells (white bars). “No RT” controls (dark gray bars) were corresponding reactions in which the RT enzyme was omitted. Each data point indicates average of duplicate determinations with error bars corresponding to the SD.
