Table 1.
Cyclin D1 mRNA Expression Normalized to β-Globin and Relative to Follicular Hyperplasia: Cell Lines
| Sample | Diagnosis | Cyclin D1 mRNA overexpression | Cyclin D1 normalized quantity* |
|---|---|---|---|
| Granta | Mantle cell lymphoma | + | 15.35 |
| NCEB | Mantle cell lymphoma | − | 0.82 |
| SUPB-15 | B-cell ALL | − | 0.30 |
| K-562 | Chronic myelogenous leukemia | − | 0.52 |
| Molt-4 | Precursor T-cell ALL | − | 0.31 |
| REH | Precursor B-cell ALL | − | 0.25 |
| 697 | Precursor B-cell ALL | − | 0.20 |
| NB-4 | Acute promyelocytic leukemia | + | 1.56 |
| Kasumi-1 | Acute myeloid leukemia | − | 0.20 |
| Karpas 299 | t(2;5) positive T-cell lymphoma | − | 0.22 |
| SW1271 | Small cell carcinoma | + | 59.30 |
| HT1080 | Fibrosarcoma | + | 290.02 |
| PA-1 | Ovary teratocarcinoma | + | 390.72 |
| SK-N-SH | Neuroblastoma | + | 18.77 |
Samples with a normalized cyclin D1 expression level greater than 1.00 were defined as overexpressing cyclin D1 MRNA.
Cyclin D1 normalized quantity = 
CT = crossing threshold.
+, positive.
−, negative.
E = efficiency of reaction.