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. 1964 Feb;44(2):215–245.

A Description of Echo-9 Virus Infection in Cultured Cells

II. Cytochemical Observations

Gabriel C Godman, Richard A Rifkind, Robert B Page, Calderon Howe, Harry M Rose
PMCID: PMC1906997  PMID: 14119168

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Selected References

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  1. ACKERMANN W. W., LOH P. C., PAYNE F. E. Studies of the biosynthesis of protein and ribonucleic acid in HeLa cells infected with poliovirus. Virology. 1959 Feb;7(2):170–183. doi: 10.1016/0042-6822(59)90185-0. [DOI] [PubMed] [Google Scholar]
  2. ANDREWES C. H., BURNET F. M., ENDERS J. F., GARD S., HIRST G. K., KAPLAN M. M., ZHDANOV V. M. Taxonomy of viruses infecting vertebrates: present knowledge and ignorance. Virology. 1961 Sep;15:52–55. doi: 10.1016/0042-6822(61)90076-9. [DOI] [PubMed] [Google Scholar]
  3. ATTARDI G., SMITH J. Virus specific protein and a ribo-nucleic acid associated with ribosomes in poliovirus infected HeLa cells. Cold Spring Harb Symp Quant Biol. 1962;27:271–292. doi: 10.1101/sqb.1962.027.001.026. [DOI] [PubMed] [Google Scholar]
  4. Alfert M., Geschwind I. I. A Selective Staining Method for the Basic Proteins of Cell Nuclei. Proc Natl Acad Sci U S A. 1953 Oct;39(10):991–999. doi: 10.1073/pnas.39.10.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BALTIMORE D., FRANKLIN R. M. Preliminary data on a virus-specific enzyme system responsible for the synthesis of viral RNA. Biochem Biophys Res Commun. 1962 Nov 27;9:388–392. doi: 10.1016/0006-291x(62)90021-9. [DOI] [PubMed] [Google Scholar]
  6. BALTIMORE D., FRANKLIN R. M. The effect of Mengovirus infection on the activity of the DNA-dependent RNA polymerase of L-cells. Proc Natl Acad Sci U S A. 1962 Aug;48:1383–1390. doi: 10.1073/pnas.48.8.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. BANGLE R., Jr The peracetic acid-Schiff stain. Am J Clin Pathol. 1954 Feb;24(2):179–185. doi: 10.1093/ajcp/24.2.179. [DOI] [PubMed] [Google Scholar]
  8. BATHER R., PURDIE-PEPPER E. Nucleic acid synthesis and transfer in normal, second generation chick embryo fibroblasts. Can J Biochem Physiol. 1961 Oct;39:1625–1633. doi: 10.1139/o61-176. [DOI] [PubMed] [Google Scholar]
  9. BECKER Y., GROSSOWICZ N., BERNKOPF H. Metabolism of human amnion cell cultures infected with poliomyelitis virus. I. Glucose metabolism during virus synthesis. Proc Soc Exp Biol Med. 1958 Jan;97(1):77–82. doi: 10.3181/00379727-97-23650. [DOI] [PubMed] [Google Scholar]
  10. BIRNS M., MASEK B. Localization of enzyme activities in frozen-substituted-fixed cells. J Histochem Cytochem. 1961 Mar;9:204–206. doi: 10.1177/9.2.204. [DOI] [PubMed] [Google Scholar]
  11. BUCKLEY S. M. Cytopathology of poliomyelitis virus in tissue culture; fluorescent antibody and tinctorial studies. Am J Pathol. 1957 Jul-Aug;33(4):691–707. [PMC free article] [PubMed] [Google Scholar]
  12. BURSTONE M. S. Histochemical demonstration of acid phosphatases with naphthol AS-phosphates. J Natl Cancer Inst. 1958 Sep;21(3):523–539. [PubMed] [Google Scholar]
  13. BURSTONE M. S. Histochemical demonstration of cytochrome oxidase with new amine reagents. J Histochem Cytochem. 1960 Jan;8:63–70. doi: 10.1177/8.1.63. [DOI] [PubMed] [Google Scholar]
  14. CORNATZER W. E., SANDSTROM W., FISCHER R. G. The effect of poliomyelitis virus type I (Mahoney strain) on the phospholipid metabolism of the HeLa cell. Biochim Biophys Acta. 1961 May 13;49:414–415. doi: 10.1016/0006-3002(61)90151-2. [DOI] [PubMed] [Google Scholar]
  15. DARNELL J. E., Jr, EAGLE H. The biosynthesis of poliovirus in cell cultures. Adv Virus Res. 1960;7:1–26. doi: 10.1016/s0065-3527(08)60004-4. [DOI] [PubMed] [Google Scholar]
  16. DARNELL J. E., Jr Early events in poliovirus infection. Cold Spring Harb Symp Quant Biol. 1962;27:149–158. doi: 10.1101/sqb.1962.027.001.017. [DOI] [PubMed] [Google Scholar]
  17. DARNELL J. E., Jr, LEVINTOW L., THOREN M. M., HOOPER J. L. The time course of synthesis of poliovirus RNA. Virology. 1961 Mar;13:271–279. doi: 10.1016/0042-6822(61)90145-3. [DOI] [PubMed] [Google Scholar]
  18. DEITCH A. D. An improved Sakaguchi reaction for microspectrophotometric use. J Histochem Cytochem. 1961 Sep;9:477–483. doi: 10.1177/9.5.477. [DOI] [PubMed] [Google Scholar]
  19. DEITCH A. D. Microspectrophotometric study of the binding of the anionic dye, naphthol yellow S, by tissue sections and by purified proteins. Lab Invest. 1955 Sep-Oct;4(5):324–351. [PubMed] [Google Scholar]
  20. DIAMOND L., BALIS M. E. A possible role of host ribonucleic acid in poliovirus synthesis. Virology. 1961 Oct;15:210–212. doi: 10.1016/0042-6822(61)90240-9. [DOI] [PubMed] [Google Scholar]
  21. EDSTROM J. E., GRAMPP W., SCHOR N. The intracellular distribution and heterogeneity of ribonucleic acid in starfish oocytes. J Biophys Biochem Cytol. 1961 Dec;11:549–557. doi: 10.1083/jcb.11.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ELFTMAN H. Phospholipid fixation by dichromate-sublimate. Stain Technol. 1957 Jan;32(1):29–31. doi: 10.3109/10520295709111393. [DOI] [PubMed] [Google Scholar]
  23. FEINENDEGEN L. E., BOND V. P., PAINTER R. B. Studies on the interrelationship of RNA synthesis, DNA synthesis and precursor pool in human tissue culture cells studied with tritiated pyrimidine nucleosides. Exp Cell Res. 1961 Jan;22:381–405. doi: 10.1016/0014-4827(61)90117-3. [DOI] [PubMed] [Google Scholar]
  24. FENWICK M. L. The influence of poliovirus infection of RNA synthesis in mammalian cells. Virology. 1963 Mar;19:241–249. doi: 10.1016/0042-6822(63)90061-8. [DOI] [PubMed] [Google Scholar]
  25. FRANKLIN R. M., BALTIMORE D. Patterns of macromolecular synthesis in normal and virus-infected mammalian cells. Cold Spring Harb Symp Quant Biol. 1962;27:175–198. doi: 10.1101/sqb.1962.027.001.019. [DOI] [PubMed] [Google Scholar]
  26. GODMAN G. C., RIFKIND R. A., HOWE C., ROSE H. M. A DESCRIPTION OF ECHO-9 VIRUS INFECTION IN CULTURED CELLS. I. THE CYTOPATHIC EFFECT. Am J Pathol. 1964 Jan;44:1–27. [PMC free article] [PubMed] [Google Scholar]
  27. Goldstein L., Plaut W. DIRECT EVIDENCE FOR NUCLEAR SYNTHESIS OF CYTOPLASMIC RIBOSE NUCLEIC ACID. Proc Natl Acad Sci U S A. 1955 Nov 15;41(11):874–880. doi: 10.1073/pnas.41.11.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. HACK M. H. A new histochemical technique for lipides applied to plasmal. Anat Rec. 1952 Feb;112(2):275–301. doi: 10.1002/ar.1091120211. [DOI] [PubMed] [Google Scholar]
  29. HAUSEN H. [Cytological studies on the growth of ME-viruses in L cells. Localization of RNA- and virus protein synthesis by autoradiographic fluorescent optical methods]. Z Naturforsch B. 1962 Mar;17B:158–160. [PubMed] [Google Scholar]
  30. HOKIN L. E., SHERWIN A. L. Protein secretion and phosphate turnover in the phospholipids in salivary glands in vitro. J Physiol. 1957 Jan 23;135(1):18–29. doi: 10.1113/jphysiol.1957.sp005690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. HOLLAND J. J. Depression of host-controlled RNA synthesis in human cells during poliovirus infection. Proc Natl Acad Sci U S A. 1963 Jan 15;49:23–28. doi: 10.1073/pnas.49.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. KOVACS E. Comparative biochemical studies on normal and poliomyelitis infected tissue cultures. IV. Enzyme-changes in host cells. Proc Soc Exp Biol Med. 1956 May;92(1):183–188. doi: 10.3181/00379727-92-22423. [DOI] [PubMed] [Google Scholar]
  33. KURNICK N. B. Methyl green-pyronin; basis of selective staining of nucleic acids. J Gen Physiol. 1950 Jan 20;33(3):243-64, pl. doi: 10.1085/jgp.33.3.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. LEBRUN J. L'antigène poliomyélitique au cours du développement intracellulaire du virus. Ann Inst Pasteur (Paris) 1957 Aug;93(2):225–229. [PubMed] [Google Scholar]
  35. LEVINTOW L., THOREN M. M., DARNELL J. E., Jr, HOOPER J. L. Effect of p-fluorophenylalanine and puromycin on the replication of poliovirus. Virology. 1962 Mar;16:220–229. doi: 10.1016/0042-6822(62)90241-6. [DOI] [PubMed] [Google Scholar]
  36. MATZELT D., HOMANN J., LENNARTZ H. Das Verhalten glykolytischer Enzymaktivitäten in Gewebekulturen vor und nach Beimpfung mit Virus. II. Messungen an Gewebekulturen, die mit Poliomyelitisvirus, Typ I, infiziert wurden, im Vergleich zu normalen Gewebekulturen. Biochem Z. 1958;330(3):260–268. [PubMed] [Google Scholar]
  37. MCMASTER-KAYE R. The metabolic characteristics of nucleolar, chromosonal, and cytoplasmic ribonucleic acid of Drosophila salivary glands. J Biophys Biochem Cytol. 1960 Oct;8:365–378. doi: 10.1083/jcb.8.2.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. MENDELSOHN M. L. The two-wavelength method of microspectrophotometry. III. An extension based on photographic color transparencies. J Biophys Biochem Cytol. 1958 Jul 25;4(4):425–431. doi: 10.1083/jcb.4.4.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. MENSCHIK Z. Nile blue histochemical method for phospholipids. Stain Technol. 1953 Jan;28(1):13–18. doi: 10.3109/10520295309112630. [DOI] [PubMed] [Google Scholar]
  40. MOORE R. D., SCHOENBERG M. D. Low temperature sulfation of tissues and the demonstration of metachromasy. Stain Technol. 1957 Sep;32(5):245–247. doi: 10.3109/10520295709111630. [DOI] [PubMed] [Google Scholar]
  41. McMASTER-KAYE R., TAYLOR J. H. Evidence for two metabolically distinct types of ribonucleic acid in chromatin and nucleoli. J Biophys Biochem Cytol. 1958 Jan 25;4(1):5–11. doi: 10.1083/jcb.4.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. NACHLAS M. M., TSOU K. C., DE SOUZA E., CHENG C. S., SELIGMAN A. M. Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J Histochem Cytochem. 1957 Jul;5(4):420–436. doi: 10.1177/5.4.420. [DOI] [PubMed] [Google Scholar]
  43. NIRENBERG M. W., MATTHAEI J. H. The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1588–1602. doi: 10.1073/pnas.47.10.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. NOVIKOFF A. B., GOLDFISCHER S. Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proc Natl Acad Sci U S A. 1961 Jun 15;47:802–810. doi: 10.1073/pnas.47.6.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. NOVIKOFF A. B., SHIN W. Y., DRUCKER J. Mitochondrial localization of oxidative enzymes: staining results with two tetrazolium salts. J Biophys Biochem Cytol. 1961 Jan;9:47–61. doi: 10.1083/jcb.9.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. PELLING G. Chromosomal synthesis of ribonucleic acid as shown by incorporation of uridine labelled with tritium. Nature. 1959 Aug 22;184(Suppl 9):655–656. doi: 10.1038/184655a0. [DOI] [PubMed] [Google Scholar]
  47. PERRY R. P. On the nucleolar and nuclear dependence of cytoplasmic RNA synthesis in HeLa cells. Exp Cell Res. 1960 Jun;20:216–220. doi: 10.1016/0014-4827(60)90240-8. [DOI] [PubMed] [Google Scholar]
  48. PRESCOTT D. M. The nuclear dependence of RNA synthesis in Acanthamoeba sp. Exp Cell Res. 1960 Feb;19:29–34. doi: 10.1016/0014-4827(60)90034-3. [DOI] [PubMed] [Google Scholar]
  49. Penman S., Scherrer K., Becker Y., Darnell J. E. POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963 May;49(5):654–662. doi: 10.1073/pnas.49.5.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. RASCH E., SWIFT H. Microphotometric analysis of the cytochemical Millon reaction. J Histochem Cytochem. 1960 Jan;8:4–17. doi: 10.1177/8.1.4. [DOI] [PubMed] [Google Scholar]
  51. REICH E., FRANKLIN R. M. Effect of mitomycin C on the growth of some animal viruses. Proc Natl Acad Sci U S A. 1961 Aug;47:1212–1217. doi: 10.1073/pnas.47.8.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. REICH E., FRANKLIN R. M., SHATKIN A. J., TATUM E. L. Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science. 1961 Aug 25;134(3478):556–557. doi: 10.1126/science.134.3478.556. [DOI] [PubMed] [Google Scholar]
  53. RIGGS J. L., LOH P. C., EVELAND W. C. A simple fractionation method for preparation of fluorescein-labeled gamma globulin. Proc Soc Exp Biol Med. 1960 Dec;105:655–658. doi: 10.3181/00379727-105-26207. [DOI] [PubMed] [Google Scholar]
  54. RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]
  55. ROTHSTEIN E. L., MANSON L. A. Phosphorus incorporation into high molecular weight RNA of poliovirus-infected HeLa cells. Virology. 1959 Sep;9:141–143. doi: 10.1016/0042-6822(59)90108-4. [DOI] [PubMed] [Google Scholar]
  56. SALZMAN N. P. Deoxyribonucleic acid, virus, and protein synthesis in animal cell cultures. Natl Cancer Inst Monogr. 1962 Apr;7:205–212. [PubMed] [Google Scholar]
  57. SALZMAN N. P., LOCKART R. Z., Jr Alteration in ribonucleic acid metabolism resulting from poliomyelitis virus infection of HeLa cells. Biochim Biophys Acta. 1959 Apr;32:572–573. doi: 10.1016/0006-3002(59)90645-6. [DOI] [PubMed] [Google Scholar]
  58. SALZMAN N. P., LOCKART R. Z., Jr, SEBRING E. D. Alterations in HeLa cell metabolism resulting from poliovirus infection. Virology. 1959 Oct;9:244–259. doi: 10.1016/0042-6822(59)90118-7. [DOI] [PubMed] [Google Scholar]
  59. SALZMAN N. P., SEBRING E. D. The source of poliovirus ribonucleic acid. Virology. 1961 Feb;13:258–260. doi: 10.1016/0042-6822(61)90062-9. [DOI] [PubMed] [Google Scholar]
  60. SIRLIN J. L. Cell sites of RNA and protein syntheses in the salivary gland of Smittia (Chironomidae). Exp Cell Res. 1960 Feb;19:177–180. doi: 10.1016/0014-4827(60)90047-1. [DOI] [PubMed] [Google Scholar]
  61. TAMAOKI T., MUELLER G. C. Synthesis of nuclear and cytoplasmic RNA of HeLa cells and the effect of actinomycin D. Biochem Biophys Res Commun. 1962 Nov 27;9:451–454. doi: 10.1016/0006-291x(62)90033-5. [DOI] [PubMed] [Google Scholar]
  62. TAYLOR-ROBINSON D., ZWARTOUW H. T., WESTWOOD J. C. The oxygen requirement for poliovirus replication in concentrated suspensions of ERK cells. Br J Exp Pathol. 1961 Aug;42:317–323. [PMC free article] [PubMed] [Google Scholar]
  63. TSUGITA A., FRAENKEL-CONRAT H., NIRENBERG M. W., MATTHAEI J. H. Demonstration of the messenger role of viral RNA. Proc Natl Acad Sci U S A. 1962 May 15;48:846–853. doi: 10.1073/pnas.48.5.846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  64. WACHSTEIN M., MEISEL E., NIEDZWIEDZ A. Histochemical demonstration of mitochondrial adenosine triphosphatase with the lead-adenosine triphosphate technique. J Histochem Cytochem. 1960 Sep;8:387–388. doi: 10.1177/8.5.387. [DOI] [PubMed] [Google Scholar]
  65. WARNER J. R., KNOPF P. M., RICH A. A multiple ribosomal structure in protein synthesis. Proc Natl Acad Sci U S A. 1963 Jan 15;49:122–129. doi: 10.1073/pnas.49.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Warner J. R., Rich A., Hall C. E. Electron Microscope Studies of Ribosomal Clusters Synthesizing Hemoglobin. Science. 1962 Dec 28;138(3548):1399–1403. doi: 10.1126/science.138.3548.1399. [DOI] [PubMed] [Google Scholar]
  67. ZIMMERMAN E. F., HEETER M., DARNELL J. E. RNA synthesis in poliovirus-infected cells. Virology. 1963 Mar;19:400–408. doi: 10.1016/0042-6822(63)90080-1. [DOI] [PubMed] [Google Scholar]

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