TABLE 2.
Genes identified in HTS of the Acinetobacter sp. strain DSM 17874 transposon mutant library
| Genea | Organism | Function or functional category | No. of mutantsb | % Identity/ length (bp) |
|---|---|---|---|---|
| ACIAD3192 | A. baylyi ADP1 | Putative monooxygenase | 1 | 81-91/35-447 |
| gacS/barA | A. baylyi ADP1 | GacS-like sensor kinase protein | 5 | 79-96/71-157 |
| xcpS | A. baylyi ADP1 | General secretion pathway protein F | 1 | 82-84/107-152 |
| xcpR | A. baylyi ADP1 | General secretion pathway protein E, putative ATPase | 2 | 87-88/61-115 |
| ACIAD0294 | A. baylyi ADP1 | General secretion pathway protein | 2 | 80/121-209 |
| 32_384 | A. baumannii AYE | Putative major facilitator superfamily drug transporter | 1 | 80/257 |
| filA | Acinetobacter sp. strain BD413 | Pilus assembly system fil gene cluster | 1 | 96/29 |
| ACIAD0505 | A. baylyi ADP1 | Formyltetrahydrofolate deformylase | 1 | 86/365 |
| ACIAD2911, panD | A. baylyi ADP1 | Aspartate 1-decarboxylase precursor CoA | 1 | 83/179 |
| ugd | Acinetobacter sp. strain RAG-1 | UDP-glucose DH (close to the wee gene cluster for emulsan production) | 1 | 100/461 |
| himD | A. baylyi ADP1 | Integration host factor, β-SU | 1 | 83/218 |
| ACIAD0049 | A. baylyi ADP1 | Putative lineoyl-CoA desaturase | 1 | 80-91/43-187 |
| ACIAD0473 | A. baylyi ADP1 | Putative trancriptional regulator (AraC family) | 2 | 83-96/26-136 |
| truA | A. baylyi ADP1 | tRNA-pseudouridine synthase | 1 | 84/150 |
| acnA | A. baylyi ADP1 | Aconitate hydratase | 1 | 81-84/242-294 |
| ACIAD3547 | A. baylyi ADP1 | Hypothetical protein, putative enzyme | 1 | 84/140 |
a
Gene with closest homology in BLASTn homology search.
b
For six mutants, there was no reliable match; five mutants could not be analyzed.