TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Specific sequence (5′ to 3′)a | Specificity (position)b |
|---|---|---|
| 8 | CAATCCTGGAAACTTTTTGGCGCT | AcveA (2021-2044) |
| 10 | ACTTTTCAGTACAACGCCAACTTTTTCCTC | AcveA (1681-1710) |
| 30 | GAGCTCCTTGTCCTGTAGCGCTTGCC | AcveA (91-110) |
| 31 | GCGGCCGCACGATATGGCTTCGGATG | AcveA (1364-1381) |
| 32 | GCGGCCGCCATCTACTCTATTCCTTTGC | hph from pZHK2 |
| 33 | TCTAGATGCCAACACATTTGCGTGCC | AcveA (3001-3020) |
| 34 | ACTAGTGGTATGGCGAGGTTACGCGT | AcveA (5069-5088) |
| 35 | TCTAGAAACGACGGCCAGTGCCAAGC | trpC(p) from pZHK2 |
| 42 | AGATCAAGACTTCGCCCTTC | AcveA (1991-2010) |
| 49 | GGCCGGCTACATGGTGAATA | AcveA (2981-3000) |
| 52 | GATCCTTGCTAGCGCCGGAC | AcveA (26-45) |
| 57 | TCTCGTTTCACCTCCACCCA | AcveA (1301-1320) |
| 59 | CAGGTATCAAAACGCAACAG | AcveA (3061-3080) |
| Ac_IPNS_U1 | ACCAGTCCGACGTGCAGAAT | pcbC gene |
| Ac_IPNS_L1 | TCGGTGATATGGGCCATGTAG | pcbC gene |
| Ac_cefEF_U1 | CCGTAACCACCAAGGGTATCT | cefEF genes |
| Ac_cefEF_L1 | CTCCTCGCTTCCGTTCTTGA | cefEF genes |
| Ac_ACVS_U1 | CGTTGCGCACCGTAACC | pcbAB genes |
| Ac_ACVS_L1 | CACTGCCTGGATAAGTCCATGA | pcbAB genes |
| Ac_cefG_U1 | AAGAGCAAACCTGCGATGGA | cefG gene |
| Ac_cefG_L1 | TCTGTGCCGTTGATTTCCTTCT | cefG gene |
| Ac_cefD1_U1 | GAACGACTTGATGAGCGGAATA | cefD1 gene |
| Ac_cefD1_L1 | TCCCCGACATAGACAAATATTGTTG | cefD1 gene |
| Ac_cefD2_U1 | CTAGCCCCAGGCCCATTCT | cefD2 gene |
| Ac_cefD2_L1 | CGATGCAGATGGACGACTTGT | cefD2 gene |
| Ac_act_U3 | GCGACGTCGATGTCCGTAA | Actin gene |
| Ac_act_L3 | AGAAGGAGCAAGAGCAGTGATCTC | Actin gene |
| 66 | CCATGGCCACCCCTTCGCTCAT | AcveA (1390-1405) |
| 74 | CCATGGCGAGCGCGGGCGGCGG | AcveA (2219-2235) |
| 75 | CCATGGTGAATACGCTGAACTT | AcveA (2971-2986) |
a
Recognition sequences for endonucleases that were used for the construction of knockout cassettes are underlined. For further details, see the text.
b
Nucleotide positions for AcveA gene are from accession no. AM410093 in the EMBL data library.