TABLE 2.
Oligonucleotide | Specific sequence (5′ to 3′)a | Specificity (position)b |
---|---|---|
8 | CAATCCTGGAAACTTTTTGGCGCT | AcveA (2021-2044) |
10 | ACTTTTCAGTACAACGCCAACTTTTTCCTC | AcveA (1681-1710) |
30 | GAGCTCCTTGTCCTGTAGCGCTTGCC | AcveA (91-110) |
31 | GCGGCCGCACGATATGGCTTCGGATG | AcveA (1364-1381) |
32 | GCGGCCGCCATCTACTCTATTCCTTTGC | hph from pZHK2 |
33 | TCTAGATGCCAACACATTTGCGTGCC | AcveA (3001-3020) |
34 | ACTAGTGGTATGGCGAGGTTACGCGT | AcveA (5069-5088) |
35 | TCTAGAAACGACGGCCAGTGCCAAGC | trpC(p) from pZHK2 |
42 | AGATCAAGACTTCGCCCTTC | AcveA (1991-2010) |
49 | GGCCGGCTACATGGTGAATA | AcveA (2981-3000) |
52 | GATCCTTGCTAGCGCCGGAC | AcveA (26-45) |
57 | TCTCGTTTCACCTCCACCCA | AcveA (1301-1320) |
59 | CAGGTATCAAAACGCAACAG | AcveA (3061-3080) |
Ac_IPNS_U1 | ACCAGTCCGACGTGCAGAAT | pcbC gene |
Ac_IPNS_L1 | TCGGTGATATGGGCCATGTAG | pcbC gene |
Ac_cefEF_U1 | CCGTAACCACCAAGGGTATCT | cefEF genes |
Ac_cefEF_L1 | CTCCTCGCTTCCGTTCTTGA | cefEF genes |
Ac_ACVS_U1 | CGTTGCGCACCGTAACC | pcbAB genes |
Ac_ACVS_L1 | CACTGCCTGGATAAGTCCATGA | pcbAB genes |
Ac_cefG_U1 | AAGAGCAAACCTGCGATGGA | cefG gene |
Ac_cefG_L1 | TCTGTGCCGTTGATTTCCTTCT | cefG gene |
Ac_cefD1_U1 | GAACGACTTGATGAGCGGAATA | cefD1 gene |
Ac_cefD1_L1 | TCCCCGACATAGACAAATATTGTTG | cefD1 gene |
Ac_cefD2_U1 | CTAGCCCCAGGCCCATTCT | cefD2 gene |
Ac_cefD2_L1 | CGATGCAGATGGACGACTTGT | cefD2 gene |
Ac_act_U3 | GCGACGTCGATGTCCGTAA | Actin gene |
Ac_act_L3 | AGAAGGAGCAAGAGCAGTGATCTC | Actin gene |
66 | CCATGGCCACCCCTTCGCTCAT | AcveA (1390-1405) |
74 | CCATGGCGAGCGCGGGCGGCGG | AcveA (2219-2235) |
75 | CCATGGTGAATACGCTGAACTT | AcveA (2971-2986) |
Recognition sequences for endonucleases that were used for the construction of knockout cassettes are underlined. For further details, see the text.
Nucleotide positions for AcveA gene are from accession no. AM410093 in the EMBL data library.