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Some C. jejuni isolates recovered from poultry do not secrete the Cia proteins. C. jejuni isolates 81-176 (lane 1), CS (lane 2), S1 (lane 3), S2B (lane 4), S3 (lane 5), SC (lane 6), and Turkey (lane 7) were incubated in MEM lacking methionine and containing 1% FBS and labeled with [35S]methionine for 3 h. The supernatant fluids were analyzed by SDS-PAGE and autoradiography as outlined in Materials and Methods. Molecular mass standards, in kDa, are indicated on the left.
