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. 2007 Mar 16;73(10):3123–3136. doi: 10.1128/AEM.01399-06

FIG. 8.

FIG. 8.

(A) The rpoN operon of the C. jejuni S2B isolate was sequenced, and a single thymine base insertion was identified between bp 1004 and 1005. This base insertion results in a termination codon at bp 1011 as opposed to the normal termination codon at bp 1248. Shown is a segment of the nucleotide sequence of the rpoN gene and its predicted translation from the C. jejuni NCTC 11168 (11168) and S2B (S2B) isolates. Also shown (bottom) is the number of bp from the start codon of rpoN. *, termination codon. (B) Complementation of the C. jejuni S2B strain with a wild-type copy of rpoN restored the motility phenotype. The C. jejuni S2B wild-type and S2B rpoN pRY111-transformed isolates were spotted onto MH plates supplemented with 0.4% agar, and motility was assessed after 48 h of incubation.