TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| MV1184 | ara-14 Δ(lac-proAB) rpsL thi-1 (φ80 lacZΔM15) Δ(srl-recA)360::Tn10 F′[traD36 proAB+lacIqlacZΔM15] | 39 |
| Pseudomonas aeruginosa | ||
| PAO1 | Prototroph | 17 |
| PAO-MW1 | lasI rhlI double mutant of PAO1 | 40 |
| PAO-NN1 | lasR mutant of PAO1 | N. Nomura |
| Plasmids | ||
| pQF50 | Broad-host-range transcriptional fusion vector; CbrlacZ IncP | 8 |
| pβ01 | pQF50 derivative containing lasB-lacZ transcriptional fusion | 18 |
| pβ02 | pQF50 derivative containing rhlA-lacZ transcriptional fusion | 18 |
| pβ03 | pQF50 derivative containing lasI-lacZ transcriptional fusion | This study |
| pMRP9-1 | Broad-host-range GFP expression vector; Cbr | 23 |
a
Cbr, carbenicillin resistant.