FIG. 4.

Cultivation and ectoine synthesis in E. coli (pASK-ectABC) in a bioreactor (1.5-liter working volume) with DM at 30°C and pH 7.0. After 22 h (marked by an arrow), anhydrotetracycline was added to induce the expression of the ectoine genes. Metabolic fluxes in the induction phase are determined for ectoine synthesis (r_ectoine = 0.040 g liter⁻¹ h⁻¹) and for the corresponding glucose consumption (r_glucose = 1.4 g liter⁻¹ h⁻¹) (curves are approximated to a linear rate).