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U. maydis strains expressing human DHODH are inhibited by brequinar. U. maydis wild-type cells (WT), pyr4 mutants (pyr4), and pyr4 mutants transformed with either full-length U. maydis DHODH (pyr4/UmDHODH) or the chimeric U. maydis-human DHODH (pyr4/Um-HsDHODH) were grown in minimal medium. Cell growth was determined 16 h after the addition of the inhibitor brequinar (10 μM).
