TABLE 1.
Electron-accepting substrates for recombinant U. maydis DHODH-ΔN
| Electron acceptor (concn, mM) | Enzyme activitya (%) |
|---|---|
| FeCy (1) | 100 |
| DCIP (1) | 90 ± 2 |
| QD (0.1) | 5 ± 1.9 |
| Q10 (0.1) | 2 ± 0.2 |
| Q6 (0.1) | 104 ± 19 |
| Q3 (0.1) | 8 ± 5 |
| Q0 (0.1) | 8 ± 9 |
| Fumarate (1) | 2 ± 0.3 |
| NAD+ (0.1) | 1 ± 0.2 |
| NADP+ (0.1) | 1 ± 0.3 |
| None | 2 ± 0.4 |
a
Enzyme activities were determined as relative initial velocities and are expressed as mean percentages (± standard errors of the means) of the activity with FeCy as the electron acceptor. All reaction mixtures contained 1 mM DHO as the substrate, and reactions were performed in the presence molecular oxygen at atmospheric pressure (equivalent to about 230 μM).