Phylogenetic tree showing the unclassified Actinobacteria group that has been implicated in N fixation in relation to the main orders within Actinobacteria. The tree was constructed from 1,343 16S rRNA positions by using maximum-likelihood analysis (PHYLIP 3.64; J. Felsenstein, Department of Genome Sciences, University of Washington, Seattle, 2005). The numbers listed above and below branches indicate bootstrap values based on 100 replications made using either parsimony (DNApars) or maximum-likelihood (DNAml) analysis, respectively (PHYLIP 3.64). Sequences from this study are in bold type. Designations which begin with S000 are Ribosomal Database Project identifiers representing sequences that are currently unclassified. The scale bar represents a sequence difference of 0.1 nucleotide per position. The letters are used to denote the different orders within the Actinobacteria as follows: A, Coriobacteriales; B, Bifidobacteriales; C, Actinomycetales; D, Acidimicrobiales; E, Rubrobacteriales. The abbreviation Mc. is used for Methanococcus, Bf. for Bifidobacterium, and My. for Mycobacterium.