TABLE 1.
Phylogenetic classification of 16S rRNA genes in clone libraries generated from DNA-SIP of soil incubated with or without 15N2
| Taxonomic designation | No. of 16S rRNA sequences for soil witha:
|
|
|---|---|---|
| 14N2 | 15N2 | |
| Alphaproteobacteria | ||
| Rhodospirillales | ||
| Unclassified Rhodospirillales | 1 | 1 |
| Rhizobiales | ||
| Methylocystis | 0 | 1 |
| Bradyrhizobium | 3 | 4 |
| Rhodoplanes | 2 | 11ψ |
| Labrys | 1 | 0 |
| Unclassified Alphaproteobacteria | 1 | 2 |
| Betaproteobacteria | ||
| Burkholderiales | ||
| Massilia | 1 | 0 |
| Ralstonia | 2 | 1 |
| Delftia | 2 | 0 |
| Insertae sedis 5 | 2 | 0 |
| Unclassified Betaproteobacteria | 0 | 5ψ |
| Gammaproteobacteria | ||
| Xanthomonadales | 1 | 1 |
| Unclassified Gammaproteobacteria | 0 | 1 |
| Deltaproteobacteria | ||
| Myxococcales | ||
| Nannocystaceae | 2 | 1 |
| Desulfuromonales | ||
| Geobacter | 2 | 0 |
| Unclassified Desulfuromonales | 1 | 0 |
| Unclassified Deltaproteobacteria | 2 | 1 |
| WS3 | ||
| Unclassified WS3 | 0 | 2 |
| Actinobacteria | ||
| Acidimicrobiales | ||
| Acidimicrobium | 2 | 2 |
| Actinomycetales | ||
| Mycobacterium | 0 | 1 |
| Propionibacterium | 1 | 1 |
| Unclassified Actinobacteria | 0 | 5ψ |
| Firmicutes | ||
| Clostridiales | ||
| Unclassified Acidaminococcaceae | 0 | 1 |
| Acidobacteria | ||
| Acidobacteriales | ||
| Acidobacterium | 2 | 2 |
| Unclassified Acidobacteriaceae | 5 | 5 |
| Planctomycetes | ||
| Planctomycetales | ||
| Isosphaera | 1 | 0 |
| Pirellula | 1 | 0 |
| Planctomyces | 3 | 0 |
| Unclassified Planctomycetaceae | 1 | 0 |
| Verrucomicrobia | ||
| Unclassified Verrucomicrobia | 2 | 1 |
| Gemmatimonadetes | ||
| Gemmatimonadales | ||
| Gemmatimonas | 1 | 0 |
| Cyanobacteria | ||
| Subsection 4 | ||
| Unclassified family 4.1 | 0 | 1 |
| Unclassified Bacteria | 2 | 1 |
a
ψ, phylogenetic groups for which the number of 16S rRNA sequences recovered in the 15N2 library is significantly greater than that observed in the control library (Fisher's exact test; P < 0.05).