TABLE 1.
Isolatea | Isolate culture ageb
|
Maturity P valuesc | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
24 h
|
48 h
|
72 h
|
|||||||||||
CFU/ml (±SD) | D10 (kGy) | r2 value | P value significance | CFU/ml (±SD) | D10 (kGy) | r2 value | P value significance | CFU/ml (±SD) | D10 (kGy) | r2 value | P value significance | ||
C9490-P | 9.18 ± 0.08 | 0.653 | 0.96 | A | 9.28 ± 0.08 | 0.494 | 0.95 | A | 9.26 ± 0.04 | 0.478 | 0.98 | A | * |
C9490-B | 7.04 ± 0.28 | 0.479 | 0.90 | B | 7.61 ± 0.40 | 0.442 | 0.80 | ACD | 7.00 ± 0.47 | 0.490 | 0.84 | A | NSD |
43894-P | 9.08 ± 0.11 | 0.479 | 0.97 | B | 8.82 ± 0.65 | 0.454 | 0.96 | AC | 8.98 ± 0.17 | 0.465 | 0.93 | A | NSD |
43894-B | 8.03 ± 0.90 | 0.385 | 0.94 | C | 7.62 ± 0.31 | 0.381 | 0.85 | D | 7.74 ± 0.30 | 0.382 | 0.94 | B | NSD |
35150-P | 9.08 ± 0.05 | 0.396 | 0.97 | C | 9.29 ± 0.06 | 0.454 | 0.99 | BC | 9.37 ± 0.05 | 0.469 | 0.99 | A | * |
35150-B | 6.59 ± 0.24 | 0.526 | 0.74 | B | 6.75 ± 0.31 | 0.464 | 0.92 | AB | 6.88 ± 0.17 | 0.503 | 0.90 | A | NSD |
Isolates were evaluated as planktonic cells (suffix “P”) or as biofilm-associated cells (suffix “B”).
Observed log CFU/ml populations are shown for control (0.0 kGy) samples, mean of 9 samples, plus or minus standard deviations (SD). r2 values of the linear regression model were used to calculate D10 values. D10 values for a given time of cultivation shown with the same letter (A, B, C, and D) are not significantly (P < 0.05) different (by analysis of covariance).
D10 values for a given isolate/cultivation method are either significantly different (*, P < 0.05) or not significantly different (NSD) across the three times of cultivation (by analysis of covariance).