TABLE 1.
Closest GenBank matches and sequence similarities of selected 16S rRNA gene-based DGGE bands
| Banda | EMBL accession no. | Closest GenBank match (accession no.) | Similarity (%) |
|---|---|---|---|
| T1 | AM085768 | Pseudomonas putida ATCC 17494 (AF094740) | 99.8 |
| T2 | AM085769 | Pseudomonas putida ATCC 17494 (AF094740) | 97.4 |
| T3 | AM085770 | Mycobacterium aurum ATCC 23366 (X55595) | 99.0 |
| T4 | AM085771 | Mycobacterium montefiorense ATCC BAA-256 (AF330038) | 98.1 |
| T5 | AM085772 | Mycobacterium montefiorense ATCC BAA-256 (AF330038) | 98.1 |
| T6 | AM085773 | Mycobacterium aurum ATCC 23366 (X55595) | 99.0 |
| T7 | AM085774 | Mycobacterium montefiorense ATCC BAA-256 (AF330038) | 98.1 |
| T8 | AM085775 | Mycobacterium montefiorense ATCC BAA-256 (AF330038) | 97.8 |
| T9 | AM236045 | Mycobacterium montefiorense ATCC BAA-256 (AF330038) | 98.5 |
a
Bands T1 to T5 were detected in a PCR of the 16S rRNA gene with eubacterial primers GC40-63f and 518r, and bands T6 to T9 were detected in a PCR of the 16S rRNA gene with Mycobacterium-specific primers MYCO66f and GC40-MYCO600r. For the positions of bands, see Fig. 4.