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. 2007 Mar 30;73(10):3380–3390. doi: 10.1128/AEM.02855-06

FIG. 4.

FIG. 4.

Adherence patterns of intermediate intestinal pathogenic E. coli strains. Adherence behavior of unconventional E. coli strains to HeLa cells and induction of actin polymerization were monitored by FAS assay. Strain 2771/97 (A; stx2 positive, estIa positive) adheres in a diffuse pattern to target cells, whereas strains 04-3908 (B; stx2 positive, estIa positive, astA positive) and 03-7355 (C; estIa positive, astA positive) exhibit aggregative adherence. Strains 4932-53 (D; LEE negative, bfp positive) and 265-1 (E; LEE negative, bfp positive) show localized adherence patterns and develop microcolonies. All strains are not able to induce actin polymerization in HeLa cells (A2, B2, C2, D2, E2). Actin filaments were labeled with phalloidin-Texas Red (red), and DNA was visualized with DAPI (blue). Bars = 10 μm.