TABLE 2.
Detection of virulence genes in E. coli by MPCR
| Pathogroup and serotype (no. of strains) | No. of strains detected by:
|
||||||
|---|---|---|---|---|---|---|---|
| MPCR targeting
|
Single-gene-specific PCR targeting
|
||||||
| uidA | escV | bfpB | astA | lifA/efa1 | ent | hly | |
| Classical EPEC | |||||||
| O55:H6 (3) | 3 | 3 | 3 | 3 | 3 | ||
| O86:H− (2) | 2 | 2 | 2 | ||||
| O86:H(rough) (1) | 1 | 1 | 1 | ||||
| O111:H− (3) | 3 | 3 | 3 | 2 | 3 | ||
| O111:H2 (2) | 2 | 2 | 2 | 2 | 2 | ||
| O111:H(NT)a (1) | 1 | 1 | 1 | 1 | 1 | ||
| O114:H2 (2) | 2 | 2 | 2 | 2 | 2 | 2 | |
| O127:H6 (1) | 1 | 1 | 1 | 1 | 1 | ||
| O128:H2 (3) | 3 | 3 | 3 | 3 | 3 | ||
| O142:H6 (2) | 2 | 2 | 2 | 2 | 2 | ||
| ATEC | |||||||
| O8:H− (1) | 1 | 1 | 1 | 1 | 1 (α-hly) | ||
| O26:H− (1) | 1 | 1 | 1 | 1 | 1 (α-hly) | ||
| O26:H11 (2) | 2 | 2 | 2 | 2 | 1 (EHEC-hly) | ||
| O26:K60 (1) | 1 | 1 | 1 | 1 | 1 (α-hly) | ||
| O55:H− (1) | 1 | 1 | 1 | 1 | |||
| O55:H6 (1) | 1 | 1 | 1 | ||||
| O55:H7 (8) | 8 | 8 | 8 | 6 | 1 (α-hly) | ||
| O86:H8:K61 (1) | 1 | 1 | |||||
| O119:H9:K61 (1) | 1 | 1 | 1 | 1 (α-hly) | |||
| O125:H− (1) | 1 | 1 | |||||
| O127:H40 (2) | 2 | 2 | 2 | ||||
| O128:H2 (3) | 3 | 3 | |||||
| O128:H8 (1) | 1 | 1 | 1 | ||||
a
NT, nontypeable.