TABLE 3.
Detection of virulence genes in E. coli by MPCR
| Pathogroup and serotype (no. of strains) | No. of strains detected by:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| MPCR targeting
|
Single-gene-specific PCR targeting
|
|||||||
| uidA | escV | stx1 | stx2 | astA | lifA/efa1 | ent | hly | |
| LEE-positive STEC | ||||||||
| O5:H− (1) | 1 | 1 | 1 | 1 (5′) | 1 | 1 (EHEC-hly) | ||
| O26:H− (1) | 1 | 1 | 1 | 1 | 1 | 1 (EHEC-hly) | ||
| O26:H11 (4) | 4 | 4 | 2 | 3 | 1 | 4 | 4 | 4 (EHEC-hly) |
| O103:H2 (2) | 2 | 2 | 2 | 2 | 2 | 2 (EHEC-hly) | ||
| O111:H− (3) | 3 | 3 | 3 | 2 | 3 | 3 | 2 (EHEC-hly) | |
| O118:H16 (1) | 1 | 1 | 1 | 1 | 1 | |||
| O145:H− (1) | 1 | 1 | 1 | 1 (5′) | 1 | 1 (EHEC-hly) | ||
| O157:H− (2) | 2 | 2 | 2 | 2 (1 [5′]) | 2 | 2 (EHEC-hly) | ||
| O157:H7 (3) | 3 | 3 | 2 | 3 | 3 (5′) | 3 | 3 (EHEC-hly) | |
| O(rough):H− (1) | 1 | 1 | stx2f | 1 | ||||
| LEE-negative STEC | ||||||||
| O8:H21 (1) | 1 | 1 | ||||||
| O23:H15 (1) | 1 | 1 | 1 | 1 | 1 (EHEC-hly) | |||
| O40:H6 (1) | 1 | 1 | 1 | |||||
| O60:H− (1) | 1 | 1 | 1 | |||||
| O76:H19 (2) | 2 | 2 | 2 | 2 (EHEC-hly) | ||||
| O77:H− (1) | 1 | 1 | 1 | |||||
| O91:H− (3) | 3 | 2 | 3 | 1 | ||||
| O91:H14 (2) | 2 | 2 | ||||||
| O101:H9 (1) | 1 | 1 | ||||||
| O113:H4 (1) | 1 | 1 | 1 | 1 | 1 (EHEC-hly) | |||
| O126:H29 (1) | 1 | 1 | ||||||
| O128:H2 (1) | 1 | 1 | 1 | 1 (EHEC-hly) | ||||
| O146:H− (1) | 1 | 1 | 1 | |||||
| O146:H21 (1) | 1 | 1 | 1 (EHEC-hly) | |||||
| O178:H19 (1) | 1 | 1 | ||||||
| O(rough):H25(1) | 1 | 1 | 1 | 1 (EHEC-hly) | ||||