TABLE 6.
Detection of virulence genes in E. coli by MPCR
| Serotype (no. of strains) of pathogroup EAEC | No. of strains detected by:
|
||||||
|---|---|---|---|---|---|---|---|
| MPCR targeting
|
Single-gene-specific PCR targeting
|
||||||
| uidA | aggR | pic | astA | lifA/efa1 | ent | hly | |
| O2:H6 (2) | 2 | 1 | 1 | 1 (α-hly) | |||
| O3:H2 (1) | 1 | 1 | 1 | 1 | 1 (α-hly) | ||
| O5:H12 (1) | 1 | 1 | 1 | ||||
| O6:H1 (1) | 1 | 1 | 1 (α-hly) | ||||
| O6:H(NT)a (1) | 1 | 1 | 1 (α-hly) | ||||
| O8:H− (1) | 1 | 1 | |||||
| O8:H25 (1) | 1 | 1 | |||||
| O17:H− (1) | 1 | 1 | 1 | ||||
| O17:H37(1) | 1 | 1 | |||||
| O18:H− (1) | 1 | 1 | 1 (α-hly) | ||||
| O21:H− (1) | 1 | 1 | |||||
| O21:H2 (1) | 1 | 1 | 1 | 1 (α-hly) | |||
| O23:H− (1) | 1 | 1 | |||||
| O25:H4 (2) | 2 | 2 | 1 (α-hly) | ||||
| O40:H18 (1) | 1 | 1 | |||||
| O44:H12:K74 (1) | 1 | 1 | 1 | ||||
| O44:H18 (1) | 1 | 1 | 1 | ||||
| O44:H(NT) (4) | 4 | 3 | 3 | 3 | 1 (α-hly) | ||
| O52:H45 (1) | 1 | 1 | |||||
| O77:H− (1) | 1 | 1 | 1 | 1 (α-hly) | |||
| O77:H11 (1) | 1 | 1 | 1 | ||||
| O78:H10 (1) | 1 | 1 | 1 | ||||
| O78:H(NT) (2) | 2 | 2 | |||||
| O86:H(NT) (3) | 3 | 3 | 1 | ||||
| O88:H4 (1) | 1 | 1 | 1 (α-hly) | ||||
| O88:H6 (1) | 1 | 1 | |||||
| O89:H− (2) | 2 | 2 | 2 | ||||
| O89:H(NT) (1) | 1 | 1 | 1 | ||||
| O92:H(NT) (1) | 1 | 1 | 1 | ||||
| O106:H− (1) | 1 | 1 | 1 | 1 | |||
| O111:H(NT) (1) | 1 | 1 | 1 | 1 | |||
| O126:H27 (1) | 1 | 1 | 1 | ||||
| O126:K71 (1) | 1 | 1 | |||||
| O126:H(NT) (3) | 3 | 1 | 1 | 3 | |||
| O127a:H4 (1) | 1 | 1 | 1 | ||||
| O127:H21 (1) | 1 | 1 | 1 | ||||
| O128:H(NT) (1) | 1 | 1 | |||||
| O130:H26 (1) | 1 | 1 | |||||
| O133:H29 (2) | 2 | 2 | |||||
| O142:K86 (1) | 1 | 1 | |||||
| O(rough):H− (1) | 1 | 1 | 1 | ||||
| O(rough):H(NT) (1) | 1 | 1 | |||||
| O(NT):H4 (1) | 1 | 1 | |||||
| O(NT):H6 (2) | 2 | 2 | |||||
a
NT, nontypeable.