TABLE 3.
DNA transfer frequencies of the bla gene on chromosome or plasmid RK2 via phage EC10a
| Location | MOI | Transductant frequency per indicated unitb
|
DNA transfer frequency per indicated unit as determined by indicated method
|
||||
|---|---|---|---|---|---|---|---|
| CFU | PFU | CPRINSc
|
DVC-CPRINSd
|
||||
| TDC | PFU | TDC | PFU | ||||
| Chromosome | 2 | (3 ± 2) × 10−9 | (1 ± 1) × 10−9 | (1 ± 0.5) × 10−3 | (7 ± 2) × 10−4 | (1 ± 0.6) × 10−3 | (5 ± 3) × 10−4 |
| 1 | 2 × 10−9 | 2 × 10−9 | (8 ± 5) × 10−4 | (8 ± 5) × 10−4 | (5 ± 3) × 10−4 | (5 ± 3) × 10−4 | |
| 0.2 | <7 × 10−10 | <4 × 10−9 | (4 ± 1) × 10−4 | (2 ± 0.6) × 10−3 | (8 ± 4) × 10−5 | (4 ± 2) × 10−4 | |
| RK2 | 2 | <2 × 10−9 | <1 × 10−9 | (1 ± 0.1) × 10−3 | 5 × 10−4 | (5 ± 3) × 10−4 | (3 ± 1) × 10−4 |
| 1 | (4 ± 7) × 10−8 | (4 ± 7) × 10−8 | (1 ± 1) × 10−3 | (1 ± 1) × 10−3 | (6 ± 5) × 10−4 | (6 ± 5) × 10−4 | |
| 0.2 | <2 × 10−9 | <1 × 10−8 | (7 ± 9) × 10−4 | (4 ± 4) × 10−3 | (2 ± 2) × 10−4 | (1 ± 1) × 10−3 | |
a
Frequencies are shown per CFU, PFU, or TDC. Values indicate means ± standard deviations for triplicate samples.
b
Transductants were incubated on LB agar medium containing ampicillin for 2 days.
c
Recipient cells carrying the bla gene were detected by CPRINS-FISH.
d
Viable cells carrying the bla gene were detected by combined DVC and CPRINS-FISH.