TABLE 3.
Assessment of rabbit and mouse antibody binding
| Protein G display construct | Quantitative ELISA assessment of rabbit IgG-binding capacity (μg IgG/mg [dry wt] of cells)a | Qualitative assessment of mouse IgG binding
|
|
|---|---|---|---|
| Labeling result by fluorescence microscopyb | Absorbance value for spectro-fluorometric assay (550-nm excitation/ 570-nm emission)c | ||
| RsaA723 (no protein G) | 0.09 (0.03) | − | 0.29 |
| RsaA723-GXS | 0.40 (0.09) | − | 0.41 (0.12) |
| RsaA944-GXS | 0.57 (0.34) | NDe | ND |
| RsaA723-GSN | 0.64 (0.19) | ++ | 0.41 (0.12) |
| RsaA723-G-M | 0.20 (0.05) | − | 0.44 (0.15) |
| RsaA723-G-M-G | 0.64 (0.32) | + | 0.39 (0.10) |
| RsaA723-G-M-G-M-G | 0.79 (0.39) | +++ | 0.65 (0.36) |
| RsaA723-M-G-M-G-M-G-M | 0.98 (0.42) | +++++ | 0.69 (0.40) |
| Pansorbind | 0.48 (0.12) | ND | ND |
a
Standard deviations are indicated in parentheses. Data represents averages of five runs.
b
−, no detectable label; +++++, maximum possible fluorescence label.
c
Values in parentheses are the result of subtraction of the RsaA723 absorbance value.
d
Pansorbin cells display protein A.
e
ND, not determined.