Skip to main content
. 2007 Mar 2;73(10):3265–3271. doi: 10.1128/AEM.02928-06

FIG. 2.

FIG. 2.

(A) Electropherogram of PCR products from wild-type (WT) T. denitrificans, the hynL mutant, and the complemented (Compl.) hynL mutant, as well as digested plasmid DNA from the complemented mutant. Lane 1, HyperLadder III, Bioline; lane 2, wild-type DNA, primers hynL-2-f and -r; lane 3, hynL mutant (strain TL001) DNA, primers hynL-2-f and -r; lane 4, complemented-mutant genomic DNA, primers hynL-2-f and -r; lane 5, complemented-mutant plasmid DNA, primers hynL-2-r and pUC19-r; lane 6, Hi-Lo Marker, Bionexus (the arrow indicates 8 kb); lane 7, complemented-mutant pTL3 plasmid DNA, NdeI digested. (B) Maps of primer positions and amplicon sizes corresponding to lanes 2 to 5 in panel A. Note that the hynL-2-f primer anneals with genomic DNA upstream of the hynL gene, whereas the pUC19-r primer anneals with pTL3 plasmid DNA, rendering these primers specific to the T. denitrificans genome and the complementation plasmid pTL3, respectively.