TABLE 1.

Ability of Stx-encoding phages from EHEC O26:H11 to lysogenize aEPEC O26:H11 and E. coli K-12 strain C600

EHEC O26 phage donora			Result (frequency) of transduction of aEPEC O26 phage recipientb,c,f						Result (frequency) of transduction of E. coli C600 recipientf
Strain (phage)e	Diagnosis or statusc,d	stx gene	10 (D)	15 (D)	22 (D)	47 (LD)	32 (D)	40 (HUS)
54(φ54)	D	stx1	—	—	—	—	—	—	C600(φ54) (1 × 10−6)
49(φ49)	D	stx1	—	—	—	—	—	—	—
51(φ51)	HUS	stx1	—	—	—	—	—	—	C600(φ51) (2 × 10−6)
C600(φH19B) (φH19B)	LS	stx1	10(φH19B) (1 × 10−7)	—	—	—	—	—	C600(φH19B) (8 × 10−5)
46(φ46)	HUS	stx2	—	—	—	47(φ46) (1 × 10−6)	32(φ46) (6 ×10−6)	—	C600(φ46) (3 × 10−5)
61(φ61)	HUS	stx2	10(φ61) (3 × 10−7)	—	—	—	32(φ61) (1 × 10−7)	—	C600(φ61) (7 × 10−6)
50(φ50)	HUS	stx2	—	15(φ50) (1 × 10−7)	22(φ50) (3 × 10−7)	—	—	40(φ50) (6 × 10−7)	C600(φ50) (4 × 10−6)

^aSix phage donors are clinical EHEC O26:H11 isolates from our laboratory; E. coli strain C600(φH19B) (45) contains stx₁-harboring phage φH19B from a clinical EHEC O26 isolate H19 (44).

^bFive phage recipients (strains 10, 15, 22, 32, and 40) are clinical aEPEC O26 isolates, all stx negative; aEPEC strain 40 was isolated from a follow-up stool of a HUS patient (patient A) whose initial stool had EHEC strain 50; strain 47 is an stx₂-negative laboratory derivative of EHEC strain 46 that fits the definition of aEPEC.

^cData are results of transduction of the respective recipient with the respective phage; lysogen designations are given as the number of the recipient strain (number of the transducing phage). Data in parentheses are rates of transduction (number of lysogens per recipient cell). —, no lysogens were identified.

^dE. coli K-12 strain C600 was a positive control for phage transduction.

^estx-harboring bacteriophages were designated by numbers of the donor strains.

^fD, diarrhea; HUS, hemolytic uremic syndrome; LD, laboratory derivative; LS, laboratory strain.