FIG. 4.

Northern blots. Two-microgram samples of total RNA were loaded per lane. (A) Northern blot filter hybridized with (a) oligonucleotide hybridizing to the untranslated region of the cel7A promoter, (b) hac1, (c) pdi1, and (d) actin. Lanes: 1, host; 2 to 9, transformants with the expression cassettes pALK1118, pALK1276, pALK1022, pALK1309, pALK1131, pALK1692, pALK1285, and pALK1502, respectively. (B) Relative signals, normalized with an actin probe. The gray and black bars represent the samples taken from the exponential growth phase and from the mycelium dry mass maximum, respectively. (a) Oligonucleotide probe. The averages from two parallel loadings are shown. The variations between the two loadings are shown with vertical lines. (b) hac1 probe. The filled bars represent the relative amounts of the full-length signals and the bars with diagonal lines represent the relative signals from the truncated hac1 mRNAs. (c) pdi1 probe.