TABLE 1.

Protein and activity data from the cultivations of single-copy Trichoderma reesei transformants producing recombinant N. flexuosa Xyn11A (35 kDa) or the truncated N. flexuosa Xyn11A polypeptide (24 kDa)^a

Strain	Expression cassette	Amt or activity
		Protein (mg/ml ± SD)	Xylanase (nkat/ml ± SD)	Mannanase (nkat/ml ± SD)	rXyn11A (mg/ml)b	rXyn11A (nmol/ml)b	Man5A carrier (nmol/ml)c
ALKO3620	None	7.9 ± 0.5	240 ± 140	110 ± 20	0	0.0
RF5724	pALK1118	6.5 ± 0.3	1,950 ± 30	160 ± 30	0.12	3.7
RF5024	pALK1276	5.4 ± 0.3	7,620 ± 620	120 ± 30	0.44	18.4
ALKO4405	pALK1022	7.1 ± 0.3	9,590 ± 540	2,890 ± 660	0.60	18.2	32.5
RF5745	pALK1692	3.3 ± 1.1	8,660 ± 340	2,850 ± 710	0.54	16.4	32.1
RF5510	pALK1309	7.2 ± 0.4	9,230 ± 420	3,020 ± 710	0.58	17.5	34.0
RF5725	pALK1131	7.2 ± 0.1	21,800 ± 3,460	5,730 ± 230	1.25	52.7d	64.5d
RF5013	pALK1285	3.2 ± 0.1	4,480 ± 530	80 ± 0.7	0.28	8.5
RF5139	pALK1502	7.4 ± 0.5	32,230 ± 890	130 ± 3.7	1.85	77.9

^aThe results are averages from two fermentations each. The cultivations were finished 94 to 96 h after inoculation, with the exception of the RF5013 (pALK1285) and RF5745 (pALK1692) cultivations that were finished after 104 and 118 h of fermentation, respectively, due to slower growth of the strains. SD, standard deviation.

^brXyn11A, recombinant 35-kDa full-length Xyn11A or the truncated 24-kDa Xyn11A polypeptide. The specific activities used in the calculations were 16,000 nkat/mg for the 35-kDa and 17,400 nkat/mg for the 24-kDa recombinant Xyn11A, according to Leskinen et al. (16). The calculated molecular masses used were 32.9 kDa and 23.8 kDa for the 35-kDa and 24-kDa xylanases, respectively. Calculated from the average activity of the two cultivations.

^cA specific activity of 2,120 nkat/mg and a molecular mass of 41.9 kDa were used in the calculations for the Man5A core-hinge (the specific activity has been determined for the Man5A core protein) (24). Calculated from the average activity of the two cultivations.

^dThe fusion protein was only partially cleaved from the Kex2 linker.