Table 1.
Precision Analysis of Competitive PCR and Real-Time Quantitation with External Standards
| Cell lines | Competitive PCR | Real-time PCR | |||
|---|---|---|---|---|---|
| Absolute | MRC-adjusted | Absolute | MRC-adjusted | ||
| Within-run | MRC-5 | 1.4 ± 0.01 | (1.0) | 0.5 ± 0.01 | (1.0) |
| T47D | 3.1 ± 0.07 | 2.3 ± 0.05 | 1.1 ± 0.03 | 2.1 ± 0.05 | |
| SKBR-3 | 15.3 ± 0.02 | 11.0 ± 0.15 | 5.6 ± 0.12 | 10.6 ± 0.23 | |
| Between-run | MRC-5 | 1.4 ± 0.02 | (1.0) | 0.5 ± 0.02 | (1.0) |
| T47D | 3.1 ± 0.08 | 2.2 ± 0.07 | 1.1 ± 0.04 | 2.0 ± 0.04 | |
| SKBR-3 | 15.9 ± 0.88 | 11.4 ± 0.40 | 5.6 ± 0.15 | 10.5 ± 0.38 | |
The mean ± standard deviation are given. The absolute HER2/neu gene dose was obtained by normalizing the HER2/neu copy number by the β-globin copy number. The MRC-5-adjusted HER2/neu gene dose, adjusting for run-to-run variance, was obtained by adjusting the absolute gene dose by the gene dose of the single copy cell line, MRC-5.