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. 2003 Nov;5(4):222–226. doi: 10.1016/S1525-1578(10)60477-1

Figure 3.

Figure 3.

Results from the ASO assay. PCR products were slot-blotted onto two nylon membranes and probed with either a wild-type or mutant radioactive probe. After incubation and washing, the membranes were exposed to film and the results were compared. Lane 1 is a no template control. Lanes 2, 4,and 5 hybridized with both the wild-type and mutant probes and are thus interpreted as heterozygous positive for the APC I1307K variant. Lanes 3 and 6 hybridized with the wild-type probe only and are thus interpreted as wild-type (negative for the I1307K variant).