Table 1.
Oligonucleotide Sequences and RT-PCR Reaction Conditions
| Assay (amplicon length) | Oligonucleotide | 5′ nt position (orientation) | Conc (nM) | Mg2+ (mM) | Sequence | Accession no. | Reference |
|---|---|---|---|---|---|---|---|
| Cyclophilin A (66 bp) | Probe | 69 (+) | 100 | 4 | CGCGTCTCCTTTGAGCTGTTTGCA | Y00052 | 24 |
| 5′ primer | 52 (+) | 300 | ACGGCGAGCCCTTGG | ||||
| 3′ primer | 117 (−) | 300 | TTTCTGCTGTCTTTGGGACCT | ||||
| Cyclin D1 (75 bp) | Probe | 358 (−) | 100 | 3 | TCTGTTCCTCGCAGACCTCCAGCA | M64349 | 25 |
| 5′ primer | 304 (+) | 200 | CCGTCCATGCGGAAGATC | ||||
| 3′ primer | 378 (−) | 200 | GAAGACCTCCTCCTCGCACT | ||||
| CD20 (73 bp) | Probe | 253 (+) | 100 | 3 | CACGCAAAGCTTCTTCATGAGGGAATCT | M27394 | 23 |
| 5′ primer | 233 (+) | 300 | ATGTCTTCACTGGTGGGCC | ||||
| 3′ primer | 305 (−) | 300 | TAATCTGGACAGCCCCCAA |