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. Author manuscript; available in PMC: 2008 Mar 2.
Published in final edited form as: J Mol Biol. 2006 Dec 22;366(4):1064–1073. doi: 10.1016/j.jmb.2006.12.047

Figure 2.

Figure 2

Lentiviral siRNA expression and reporter system. (a) Schematic presentation of the reporter system that was used for the screening of effective siRNA targeting KLF factors. The luciferase reporter gene and the target KLF gene were expressed bicistronically under the CMV promoter. (b) Lentiviral vectors for siRNA expression. It expresses the siRNA under the human U6 promoter. It also expresses the GFP and Neo genes under a CMV promoter bicistronically. Transduced cells can be monitored by GFP expression and selected by GFP sorting or G418 resistance.