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. Author manuscript; available in PMC: 2008 Mar 2.
Published in final edited form as: J Mol Biol. 2006 Dec 22;366(4):1064–1073. doi: 10.1016/j.jmb.2006.12.047

Figure 4.

Figure 4

Figure 4

Knockdown of Endogenous Sp/KLF factors by RNAi. (a) Endogenous knockdown of Sp/KLF factors in K562 cells. Lentiviral vector expressing siRNA targeting Sp/KLF factors were used to transduce K562 cells. Total RNA were isolated 2 weeks later. The expression of endogenous Sp/KLF factors following transduction with lentiviruses expressing siRNA targeting a KLF factor or control siRNA targeting luciferase was determined using RT-PCR with GAPDH as an internal control. (b) Quantification of the data shown in (a). The data are presented as the percentage of mRNA level in cells transduced with lentiviruses expressing siRNA targeting the KLF factor and cells transduced with lentiviruses expressing luciferase control siRNA after normalization with the GAPDH internal control.