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. Author manuscript; available in PMC: 2008 Mar 2.
Published in final edited form as: J Mol Biol. 2006 Dec 22;366(4):1064–1073. doi: 10.1016/j.jmb.2006.12.047

Figure 6.

Figure 6

Endogenous knockdown of Sp1 in K562 cells and the phenotypic changes of the cells. (a) Western Blot analysis using anti-Sp1 antibody showed a decrease of Sp1 protein in K562 cells transduced with lentivirus expressing Sp1 siRNA. The decrease of Sp1 protein is comparable to that of Sp1 transcripts as demonstrated by RT-PCR. (b) and (c) Phenotypic change of K562 cells from white to red when Sp1 is silenced. The redness is correlated with the effectiveness of the Sp1 siRNA. Sp1 siRNA-1 is more potent than Sp1 siRNA-2 in Sp1 knockdown, therefore, the redness of K562 cells transduced with the former are darker than that transduced with the latter.