Figure 3.

Effect of delay in freezing on the expression of mRNA for MART-1. Ten samples from each of four different patients (n = 4) were obtained from melanoma lymph node metastases. Data are expressed as a ratio between arbitrary units of MART-1 mRNA and β-actin mRNA. The measurement of MART-1 and β-actin mRNA levels are described in the Materials and Methods. Amplicon sizes for MART-1 and β-actin were 136 bp and 99 bp, respectively. Each point represents the mean of paired samples measured in duplicate. Vertical lines represent minimum and maximum measurement.