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. 1992 May;106(1):95–100. doi: 10.1111/j.1476-5381.1992.tb14299.x

Comparison of the contractile effects of endothelin-1 and sarafotoxin S6b in goat isolated cerebral arteries.

J B Salom 1, G Torregrosa 1, F J Miranda 1, J A Alabadí 1, E Alborch 1
PMCID: PMC1907440  PMID: 1380386

Abstract

1. The effects of endothelium-derived endothelin-1 and snake venom-derived sarafotoxin S6b, peptides with striking structural and functional similarities, were examined and compared in isolated middle cerebral arteries of goats. 2. Endothelin-1 and sarafotoxin S6b contracted cerebral arteries in a concentration-dependent manner. The potency of endothelin-1 (EC50 = 4.9 (3.9-6.2) x 10(-10) M) was about ten times higher than that of sarafotoxin S6b (EC50 = 5.5 (4.4-6.9) x 10(-9) M). The tension returned to basal values after repeated washings and contraction with endothelin-1 could be reproduced. Endothelin-1 and sarafotoxin S6b induced further contraction in arteries precontracted with prostaglandin F2 alpha (10(-5) M). 3. Mechanical removal of the endothelium or incubation with indomethacin (10(-5) M) displaced the concentration-response curves to endothelin-1 and, more pronouncedly, to sarafotoxin S6b to the left. The maximum response to sarafotoxin S6b was also increased by either of these two treatments. 4. Incubation in 'nominally' Ca(2+)-free medium attenuated the vasoconstrictor response to endothelin-1 but not to sarafotoxin S6b, which was inhibited after incubation in Ca(2+)-free medium to which EGTA (10(-4) M) had been added. Pretreatment with caffeine (2 x 10(-2) M) in Ca(2+)-free medium abolished responses to endothelin-1 and sarafotoxin S6b. 5. Bay K 8644 (10(-10) M, 10(-8) M) enhanced and nicardipine (10(-10) M, 10(-8) M) inhibited in a concentration-dependent manner vasoconstrictor response to endothelin-1. Response to sarafotoxin S6b was only affected by 10(-8) M Bay K 8644 or nicardipine.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Asano T., Ikegaki I., Suzuki Y., Satoh S., Shibuya M. Endothelin and the production of cerebral vasospasm in dogs. Biochem Biophys Res Commun. 1989 Mar 31;159(3):1345–1351. doi: 10.1016/0006-291x(89)92258-4. [DOI] [PubMed] [Google Scholar]
  2. Auguet M., Delaflotte S., Chabrier P. E., Pirotzky E., Clostre F., Braquet P. Endothelin and Ca++ agonist Bay K 8644: different vasoconstrictive properties. Biochem Biophys Res Commun. 1988 Oct 14;156(1):186–192. doi: 10.1016/s0006-291x(88)80822-2. [DOI] [PubMed] [Google Scholar]
  3. Auguet M., Delaflotte S., Guillon J. M., Roubert P., Chabrier P. E., Braquet P. Endothelin-1, but not sarafotoxin s6b, induces cross-desensitization of rat aorta contraction. Eur J Pharmacol. 1990 Oct 9;187(2):293–294. doi: 10.1016/0014-2999(90)90017-z. [DOI] [PubMed] [Google Scholar]
  4. Bousso-Mittler D., Kloog Y., Wollberg Z., Bdolah A., Kochva E., Sokolovsky M. Functional endothelin/sarafotoxin receptors in the rat uterus. Biochem Biophys Res Commun. 1989 Aug 15;162(3):952–957. doi: 10.1016/0006-291x(89)90765-1. [DOI] [PubMed] [Google Scholar]
  5. Caplan B. A., Schwartz C. J. Increased endothelial cell turnover in areas of in vivo Evans Blue uptake in the pig aorta. Atherosclerosis. 1973 May-Jun;17(3):401–417. doi: 10.1016/0021-9150(73)90031-2. [DOI] [PubMed] [Google Scholar]
  6. Douglas S. A., Hiley C. R. Endothelium-dependent vascular activities of endothelin-like peptides in the isolated superior mesenteric arterial bed of the rat. Br J Pharmacol. 1990 Sep;101(1):81–88. doi: 10.1111/j.1476-5381.1990.tb12093.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edwards R., Trizna W. Response of isolated intracerebral arterioles to endothelins. Pharmacology. 1990;41(3):149–152. doi: 10.1159/000138711. [DOI] [PubMed] [Google Scholar]
  8. Eglen R. M., Michel A. D., Sharif N. A., Swank S. R., Whiting R. L. The pharmacological properties of the peptide, endothelin. Br J Pharmacol. 1989 Aug;97(4):1297–1307. doi: 10.1111/j.1476-5381.1989.tb12592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Faraci F. M. Effects of endothelin and vasopressin on cerebral blood vessels. Am J Physiol. 1989 Sep;257(3 Pt 2):H799–H803. doi: 10.1152/ajpheart.1989.257.3.H799. [DOI] [PubMed] [Google Scholar]
  10. Goto K., Kasuya Y., Matsuki N., Takuwa Y., Kurihara H., Ishikawa T., Kimura S., Yanagisawa M., Masaki T. Endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in vascular smooth muscle. Proc Natl Acad Sci U S A. 1989 May;86(10):3915–3918. doi: 10.1073/pnas.86.10.3915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Han S. P., Knuepfer M. M., Trapani A. J., Fok K. F., Westfall T. C. Cardiac and vascular actions of sarafotoxin S6b and endothelin-1. Life Sci. 1990;46(11):767–775. doi: 10.1016/0024-3205(90)90064-x. [DOI] [PubMed] [Google Scholar]
  12. Han S. P., Knuepfer M. M., Trapani A. J., Fok K. F., Westfall T. C. Endothelin and sarafotoxin S6b have similar vasoconstrictor effects and postsynaptically mediated mechanisms. Eur J Pharmacol. 1990 Feb 20;177(1-2):29–34. doi: 10.1016/0014-2999(90)90546-i. [DOI] [PubMed] [Google Scholar]
  13. Han S. P., Trapani A. J., Fok K. F., Westfall T. C., Knuepfer M. M. A comparison of the hemodynamic effects of endothelin-1 and sarafotoxin S6b in conscious rats. J Pharmacol Exp Ther. 1991 Jan;256(1):183–188. [PubMed] [Google Scholar]
  14. Hardebo J. E., Kåhrström J., Owman C., Salford L. G. Endothelin is a potent constrictor of human intracranial arteries and veins. Blood Vessels. 1989;26(5):249–253. doi: 10.1159/000158774. [DOI] [PubMed] [Google Scholar]
  15. Hirata Y., Yoshimi H., Marumo F., Watanabe T. X., Kumagaye S., Nakajima K., Kimura T., Sakakibara S. Interaction of synthetic sarafotoxin with rat vascular endothelin receptors. Biochem Biophys Res Commun. 1989 Jul 14;162(1):441–447. doi: 10.1016/0006-291x(89)92017-2. [DOI] [PubMed] [Google Scholar]
  16. Jansen I., Fallgren B., Edvinsson L. Mechanisms of action of endothelin on isolated feline cerebral arteries: in vitro pharmacology and electrophysiology. J Cereb Blood Flow Metab. 1989 Dec;9(6):743–747. doi: 10.1038/jcbfm.1989.106. [DOI] [PubMed] [Google Scholar]
  17. Kasuya Y., Ishikawa T., Yanagisawa M., Kimura S., Goto K., Masaki T. Mechanism of contraction to endothelin in isolated porcine coronary artery. Am J Physiol. 1989 Dec;257(6 Pt 2):H1828–H1835. doi: 10.1152/ajpheart.1989.257.6.H1828. [DOI] [PubMed] [Google Scholar]
  18. Kasuya Y., Takuwa Y., Yanagisawa M., Kimura S., Goto K., Masaki T. Endothelin-1 induces vasoconstriction through two functionally distinct pathways in porcine coronary artery: contribution of phosphoinositide turnover. Biochem Biophys Res Commun. 1989 Jun 30;161(3):1049–1055. doi: 10.1016/0006-291x(89)91349-1. [DOI] [PubMed] [Google Scholar]
  19. Kauser K., Rubanyi G. M., Harder D. R. Endothelium-dependent modulation of endothelin-induced vasoconstriction and membrane depolarization in cat cerebral arteries. J Pharmacol Exp Ther. 1990 Jan;252(1):93–97. [PubMed] [Google Scholar]
  20. Kloog Y., Bousso-Mittler D., Bdolah A., Sokolovsky M. Three apparent receptor subtypes for the endothelin/sarafotoxin family. FEBS Lett. 1989 Aug 14;253(1-2):199–202. doi: 10.1016/0014-5793(89)80958-5. [DOI] [PubMed] [Google Scholar]
  21. Kloog Y., Sokolovsky M. Similarities in mode and sites of action of sarafotoxins and endothelins. Trends Pharmacol Sci. 1989 Jun;10(6):212–214. doi: 10.1016/0165-6147(89)90261-7. [DOI] [PubMed] [Google Scholar]
  22. Kodama M., Kanaide H., Abe S., Hirano K., Kai H., Nakamura M. Endothelin-induced Ca-independent contraction of the porcine coronary artery. Biochem Biophys Res Commun. 1989 May 15;160(3):1302–1308. doi: 10.1016/s0006-291x(89)80145-7. [DOI] [PubMed] [Google Scholar]
  23. Koseki C., Imai M., Hirata Y., Yanagisawa M., Masaki T. Autoradiographic distribution in rat tissues of binding sites for endothelin: a neuropeptide? Am J Physiol. 1989 Apr;256(4 Pt 2):R858–R866. doi: 10.1152/ajpregu.1989.256.4.R858. [DOI] [PubMed] [Google Scholar]
  24. Landan G., Bdolah A., Wollberg Z., Kochva E., Graur D. Evolution of the sarafotoxin/endothelin superfamily of proteins. Toxicon. 1991;29(2):237–244. doi: 10.1016/0041-0101(91)90108-4. [DOI] [PubMed] [Google Scholar]
  25. Lee S. Y., Lee C. Y., Chen Y. M., Kochva E. Coronary vasospasm as the primary cause of death due to the venom of the burrowing asp, Atractaspis engaddensis. Toxicon. 1986;24(3):285–291. doi: 10.1016/0041-0101(86)90153-4. [DOI] [PubMed] [Google Scholar]
  26. Maggi C. A., Giuliani S., Patacchini R., Santicioli P., Giachetti A., Meli A. Further studies on the response of the guinea-pig isolated bronchus to endothelins and sarafotoxin S6b. Eur J Pharmacol. 1990 Jan 25;176(1):1–9. doi: 10.1016/0014-2999(90)90126-q. [DOI] [PubMed] [Google Scholar]
  27. Marsault R., Vigne P., Breittmayer J. P., Frelin C. Astrocytes are target cells for endothelins and sarafotoxin. J Neurochem. 1990 Jun;54(6):2142–2144. doi: 10.1111/j.1471-4159.1990.tb04921.x. [DOI] [PubMed] [Google Scholar]
  28. Miura K., Yukimura T., Yamashita Y., Shimmen T., Okumura M., Yamanaka S., Imanishi M., Yamamoto K. Renal and femoral vascular responses to endothelin-1 in dogs: role of prostaglandins. J Pharmacol Exp Ther. 1991 Jan;256(1):11–17. [PubMed] [Google Scholar]
  29. Neubauer S., Ertl G., Haas U., Pulzer F., Kochsiek K. Effects of endothelin-1 in isolated perfused rat heart. J Cardiovasc Pharmacol. 1990 Jul;16(1):1–8. doi: 10.1097/00005344-199007000-00001. [DOI] [PubMed] [Google Scholar]
  30. Ohlstein E. H., Horohonich S., Hay D. W. Cellular mechanisms of endothelin in rabbit aorta. J Pharmacol Exp Ther. 1989 Aug;250(2):548–555. [PubMed] [Google Scholar]
  31. Papadopoulos S. M., Gilbert L. L., Webb R. C., D'Amato C. J. Characterization of contractile responses to endothelin in human cerebral arteries: implications for cerebral vasospasm. Neurosurgery. 1990 May;26(5):810–815. doi: 10.1097/00006123-199005000-00013. [DOI] [PubMed] [Google Scholar]
  32. Rakugi H., Nakamaru M., Tabuchi Y., Nagano M., Mikami H., Ogihara T. Endothelin stimulates the release of prostacyclin from rat mesenteric arteries. Biochem Biophys Res Commun. 1989 Apr 28;160(2):924–928. doi: 10.1016/0006-291x(89)92523-0. [DOI] [PubMed] [Google Scholar]
  33. Randall M. D., Douglas S. A., Hiley C. R. Vascular activities of endothelin-1 and some alanyl substituted analogues in resistance beds of the rat. Br J Pharmacol. 1989 Oct;98(2):685–699. doi: 10.1111/j.1476-5381.1989.tb12644.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Saito A., Shiba R., Kimura S., Yanagisawa M., Goto K., Masaki T. Vasoconstrictor response of large cerebral arteries of cats to endothelin, an endothelium-derived vasoactive peptide. Eur J Pharmacol. 1989 Mar 21;162(2):353–358. doi: 10.1016/0014-2999(89)90299-9. [DOI] [PubMed] [Google Scholar]
  35. Saito A., Shiba R., Yanagisawa M., Masaki T., Kimura S., Yamada K., Mima T., Shigeno T., Goto K. Endothelins: vasoconstrictor effects and localization in canine cerebral arteries. Br J Pharmacol. 1991 May;103(1):1129–1135. doi: 10.1111/j.1476-5381.1991.tb12312.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Sakata K., Ozaki H., Kwon S. C., Karaki H. Effects of endothelin on the mechanical activity and cytosolic calcium level of various types of smooth muscle. Br J Pharmacol. 1989 Oct;98(2):483–492. doi: 10.1111/j.1476-5381.1989.tb12621.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Salom J. B., Torregrosa G., Miranda F. J., Alabadí J. A., Alvarez C., Alborch E. Ca2+ entry blockers inhibit prostaglandin F2 alpha-induced cerebrovascular contractile responses in goats. Eur J Pharmacol. 1991 Oct 2;203(1):33–39. doi: 10.1016/0014-2999(91)90787-q. [DOI] [PubMed] [Google Scholar]
  38. Salom J. B., Torregrosa G., Miranda F. J., Alabadí J. A., Alvarez C., Alborch E. Effects of Ca2+ entry blockers on CaCl2-, KCl- and noradrenaline-induced contractions of goat cerebral arteries. Eur J Pharmacol. 1990 Feb 20;177(1-2):1–8. doi: 10.1016/0014-2999(90)90543-f. [DOI] [PubMed] [Google Scholar]
  39. Salom J. B., Torregrosa G., Miranda F. J., Alabadí J. A., Alvarez C., Alborch E. Effects of endothelin-1 on the cerebrovascular bed of the goat. Eur J Pharmacol. 1991 Jan 3;192(1):39–45. doi: 10.1016/0014-2999(91)90066-y. [DOI] [PubMed] [Google Scholar]
  40. Tabrizchi R., Triggle C. R. Effects of nifedipine, BAY K 8644, and pertussis toxin on pressor response to sarafotoxin-b in pithed rats. J Cardiovasc Pharmacol. 1990 Sep;16(3):495–500. doi: 10.1097/00005344-199009000-00021. [DOI] [PubMed] [Google Scholar]
  41. Tanoi C., Suzuki Y., Shibuya M., Sugita K., Masuzawa K., Asano M. Mechanism of the enhanced vasoconstrictor responses to endothelin-1 in canine cerebral arteries. J Cereb Blood Flow Metab. 1991 May;11(3):371–379. doi: 10.1038/jcbfm.1991.77. [DOI] [PubMed] [Google Scholar]
  42. Topouzis S., Huggins J. P., Pelton J. T., Miller R. C. Modulation by endothelium of the responses induced by endothelin-1 and by some of its analogues in rat isolated aorta. Br J Pharmacol. 1991 Feb;102(2):545–549. doi: 10.1111/j.1476-5381.1991.tb12208.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Vanhoutte P. M., Rubanyi G. M., Miller V. M., Houston D. S. Modulation of vascular smooth muscle contraction by the endothelium. Annu Rev Physiol. 1986;48:307–320. doi: 10.1146/annurev.ph.48.030186.001515. [DOI] [PubMed] [Google Scholar]
  44. Warner T. D., de Nucci G., Vane J. R. Rat endothelin is a vasodilator in the isolated perfused mesentery of the rat. Eur J Pharmacol. 1989 Jan 17;159(3):325–326. doi: 10.1016/0014-2999(89)90167-2. [DOI] [PubMed] [Google Scholar]
  45. Yanagisawa M., Kurihara H., Kimura S., Tomobe Y., Kobayashi M., Mitsui Y., Yazaki Y., Goto K., Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988 Mar 31;332(6163):411–415. doi: 10.1038/332411a0. [DOI] [PubMed] [Google Scholar]
  46. Yanagisawa M., Masaki T. Endothelin, a novel endothelium-derived peptide. Pharmacological activities, regulation and possible roles in cardiovascular control. Biochem Pharmacol. 1989 Jun 15;38(12):1877–1883. doi: 10.1016/0006-2952(89)90484-x. [DOI] [PubMed] [Google Scholar]
  47. de Aguilera E. M., Irurzun A., Vila J. M., Aldasoro M., Galeote M. S., Lluch S. Role of endothelium and calcium channels in endothelin-induced contraction of human cerebral arteries. Br J Pharmacol. 1990 Mar;99(3):439–440. doi: 10.1111/j.1476-5381.1990.tb12945.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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