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Separation of supercoiled DNA plasmids by capillary electrophoresis in Pluronic gels. The standard (Promega) contains nine plasmid DNAs from 2 to 10 kb in length, differing by 1 kb. The 5-kb plasmid was supplied at about 3–4 times the abundance of other species. Optimal resolution (shown) was obtained in 30% Pluronic F127 (25°C; gradient, 500 V/cm; column length, 36 cm). (Inset) Low-resolution electrophoresis in 25% Pluronic F127 (25°C; gradient, 300 V/cm; column length, 7 cm).
