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. 1993 Jan;108(1):79–84. doi: 10.1111/j.1476-5381.1993.tb13443.x

Sympathetic innervation and alpha-adrenoceptor profile of blood vessels in the posterior region of the rabbit knee joint.

H Najafipour 1, W R Ferrell 1
PMCID: PMC1907731  PMID: 8094028

Abstract

1. Experiments were performed to determine the nature of adrenoceptors mediating neurally-induced vasoconstriction of blood vessels in the posterior region of the rabbit knee joint capsule. 2. Electrical stimulation of the posterior articular nerve resulted in frequency-dependent vasoconstriction which was maximal at 10 Hz. This response was mediated predominantly by alpha 2-adrenoceptors as it was only slightly reduced by prazosin administration and was not only abolished but converted into a dilator response by the alpha 2-adrenoceptor antagonist rauwolscine. Further experiments with another specific alpha 1-adrenoceptor antagonist YM-12617 showed that the frequency-response curve in the presence of this antagonist did not differ significantly from control. 3. Neurally-induced vasoconstriction did not appear to have a purinergic component as it was unaffected by the P2x-purinoceptor desensitiser alpha, beta methylene ATP. 4. The rank-order of potency of alpha-adrenoceptor agonists given as a bolus by close intra-arterial injection was: adrenaline = UK-14304 > clonidine > phenylephrine, suggesting that the vasoconstrictor effects were mediated predominantly by postjunctional alpha 2-adrenoceptors. 5. The alpha 2-adrenoceptor antagonist rauwolscine converted the constrictor response to close intra-arterial injection of adrenaline into a dilator response. The vasoconstrictor responses to UK-14304, clonidine and phenylephrine were substantially inhibited by rauwolscine. The alpha 1-adrenoceptor antagonist prazosin failed to inhibit the vasoconstrictor responses to adrenaline, clonidine and UK-14304 and resulted in enhancement of their constrictor effects.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. COBBOLD A. F., LEWIS O. J. The action of adrenaline, noradrenaline and acetylcholine on blood flow through joints. J Physiol. 1956 Aug 28;133(2):472–474. doi: 10.1113/jphysiol.1956.sp005602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COBBOLD A. F., LEWIS O. J. The nervous control of joint blood vessels. J Physiol. 1956 Aug 28;133(2):467–471. doi: 10.1113/jphysiol.1956.sp005601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Docherty J. R., Hyland L. Evidence for neuro-effector transmission through postjunctional alpha 2-adrenoceptors in human saphenous vein. Br J Pharmacol. 1985 Feb;84(2):573–576. doi: 10.1111/j.1476-5381.1985.tb12942.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Docherty J. R. The pharmacology of alpha 1- and alpha 2-adrenoceptors: evidence for and against a further subdivision. Pharmacol Ther. 1989;44(2):241–284. doi: 10.1016/0163-7258(89)90067-3. [DOI] [PubMed] [Google Scholar]
  5. Dunn W. R., McGrath J. C., Wilson V. G. Influence of angiotensin II on the alpha-adrenoceptors involved in mediating the response to sympathetic nerve stimulation in the rabbit isolated distal saphenous artery. Br J Pharmacol. 1991 Jan;102(1):10–12. doi: 10.1111/j.1476-5381.1991.tb12123.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ferrell W. R., Khoshbaten A., Angerson W. J. Responses of bone and joint blood vessels in cats and rabbits to electrical stimulation of nerves supplying the knee. J Physiol. 1990 Dec;431:677–687. doi: 10.1113/jphysiol.1990.sp018354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferrell W. R., Khoshbaten A. Responses of blood vessels in the rabbit knee to electrical stimulation of the joint capsule. J Physiol. 1990 Apr;423:569–578. doi: 10.1113/jphysiol.1990.sp018040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ferrell W. R., Khoshbaten A. The role of the endothelium in mediating the actions of ATP, adenosine and acetylcholine on flow through blood vessels in the rabbit knee joint. Br J Pharmacol. 1990 Feb;99(2):379–383. doi: 10.1111/j.1476-5381.1990.tb14712.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferrell W. R., Najafipour H. Changes in synovial PO2 and blood flow in the rabbit knee joint due to stimulation of the posterior articular nerve. J Physiol. 1992 Apr;449:607–617. doi: 10.1113/jphysiol.1992.sp019104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hedlund H., Fändriks L., Delbro D., Fasth S. Blockade of non-cholinergic non-adrenergic colonic contraction in response to pelvic nerve stimulation by large doses of alpha, beta-methylene ATP. Acta Physiol Scand. 1983 Dec;119(4):451–454. doi: 10.1111/j.1748-1716.1983.tb07361.x. [DOI] [PubMed] [Google Scholar]
  11. Honda K., Takenaka T., Miyata-Osawa A., Terai M., Shiono K. Studies on YM-12617: a selective and potent antagonist of postsynaptic alpha 1-adrenoceptors. Naunyn Schmiedebergs Arch Pharmacol. 1985 Jan;328(3):264–272. doi: 10.1007/BF00515552. [DOI] [PubMed] [Google Scholar]
  12. Kasakov L., Burnstock G. The use of the slowly degradable analog, alpha, beta-methylene ATP, to produce desensitisation of the P2-purinoceptor: effect on non-adrenergic, non-cholinergic responses of the guinea-pig urinary bladder. Eur J Pharmacol. 1982 Dec 24;86(2):291–294. doi: 10.1016/0014-2999(82)90330-2. [DOI] [PubMed] [Google Scholar]
  13. Khoshbaten A., Ferrell W. R. Alterations in cat knee joint blood flow induced by electrical stimulation of articular afferents and efferents. J Physiol. 1990 Nov;430:77–86. doi: 10.1113/jphysiol.1990.sp018282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Komori S., Kwon S. C., Ohashi H. Effects of prolonged exposure to alpha,beta-methylene ATP on non-adrenergic, non-cholinergic excitatory transmission in the rectum of the chicken. Br J Pharmacol. 1988 May;94(1):9–18. doi: 10.1111/j.1476-5381.1988.tb11494.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McGrath J. C., Brown C. M., Wilson V. G. Alpha-adrenoceptors: a critical review. Med Res Rev. 1989 Oct-Dec;9(4):407–533. doi: 10.1002/med.2610090403. [DOI] [PubMed] [Google Scholar]
  16. Sneddon P., Burnstock G. Inhibition of excitatory junction potentials in guinea-pig vas deferens by alpha, beta-methylene-ATP: further evidence for ATP and noradrenaline as cotransmitters. Eur J Pharmacol. 1984 Apr 13;100(1):85–90. doi: 10.1016/0014-2999(84)90318-2. [DOI] [PubMed] [Google Scholar]

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