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Acetylation state of histones used. Histone isoforms purified from TSA-treated (Acetyl.) or untreated (Control) CV1 cells were separated on Triton/acid/urea gels. The isoform distribution of input histones (In) is contrasted with the status of histones after incorporation into chromatin (On). For histone H4 the familiar isoform distribution from unacetylated (0) to tetraacetylated H4 (4) is marked to the right side of the gel.
