Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1991 Jun;103(2):1367–1372. doi: 10.1111/j.1476-5381.1991.tb09795.x

Human interleukin-1 induces a rapid relaxation of the rabbit isolated mesenteric artery.

F Marceau 1, E Petitclerc 1, D DeBlois 1, P Pradelles 1, P E Poubelle 1
PMCID: PMC1908368  PMID: 1884096

Abstract

1. Strips of rabbit superior mesenteric artery, precontracted with phenylephrine, relaxed when exposed to human recombinant interleukin-1 (IL-1) of the alpha or beta types. The effect was observed within 10 min, was optimal 32 min after the application of the cytokines and concentration-dependent (12-290 pM). 2. IL-1 alpha and IL-1 beta were equipotent in relaxing the rabbit mesenteric artery. A synthetic fragment corresponding to IL-1 beta 163-171 was approximately one million fold less active than IL-1 beta. The tripeptide Lys-D-Pro-Thr, an analogue of IL-1 beta 193-195, was inactive as an antagonist of IL-1 beta on the preparation. 3. Indomethacin (2.8 microM) prevented or acutely reversed IL-1-induced relaxations in the rabbit mesenteric artery. Purified haemoglobin (10 microM) or the removal of endothelium had no effect on relaxations elicited by IL-1 beta. 4. The preparation exhibited some selectivity for IL-1 as recombinant human tumour necrosis factor-alpha (TNF-alpha), IL-2 or IL-6 failed to influence it. TNF-alpha was not synergistic with a subthreshold concentration of IL-1 beta. 5. Immunoreactive 6-keto-prostaglandin F1 alpha and prostaglandin E2 were increased in the bathing fluid of isolated mesenteric arteries exposed to IL-1 beta as compared to controls. 6. A supernatant of lipopolysaccharide-stimulated human monocytes produced a relaxation of the preparation with a profile similar to that produced with IL-1s and there was a good quantitative agreement between the extent of the relaxation and the enzyme immunoassay measurements of IL-1 alpha and IL-1 beta in the supernatant.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
1367

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antoni G., Presentini R., Perin F., Tagliabue A., Ghiara P., Censini S., Volpini G., Villa L., Boraschi D. A short synthetic peptide fragment of human interleukin 1 with immunostimulatory but not inflammatory activity. J Immunol. 1986 Nov 15;137(10):3201–3204. [PubMed] [Google Scholar]
  2. Boraschi D., Nencioni L., Villa L., Censini S., Bossù P., Ghiara P., Presentini R., Perin F., Frasca D., Doria G. In vivo stimulation and restoration of the immune response by the noninflammatory fragment 163-171 of human interleukin 1 beta. J Exp Med. 1988 Aug 1;168(2):675–686. doi: 10.1084/jem.168.2.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burch R. M., Connor J. R., Axelrod J. Interleukin 1 amplifies receptor-mediated activation of phospholipase A2 in 3T3 fibroblasts. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6306–6309. doi: 10.1073/pnas.85.17.6306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cannon J. G., Clark B. D., Wingfield P., Schmeissner U., Losberger C., Dinarello C. A., Shaw A. R. Rabbit IL-1. Cloning, expression, biologic properties, and transcription during endotoxemia. J Immunol. 1989 Apr 1;142(7):2299–2306. [PubMed] [Google Scholar]
  5. Churchill L., Ward P. E. Relaxation of isolated mesenteric arteries by des-Arg9-bradykinin stimulation of B1 receptors. Eur J Pharmacol. 1986 Oct 14;130(1-2):11–18. doi: 10.1016/0014-2999(86)90178-0. [DOI] [PubMed] [Google Scholar]
  6. Deblois D., Marceau F. The ability of des-Arg9-bradykinin to relax rabbit isolated mesenteric arteries is acquired during in vitro incubation. Eur J Pharmacol. 1987 Oct 6;142(1):141–144. doi: 10.1016/0014-2999(87)90664-9. [DOI] [PubMed] [Google Scholar]
  7. Ferreira S. H., Lorenzetti B. B., Bristow A. F., Poole S. Interleukin-1 beta as a potent hyperalgesic agent antagonized by a tripeptide analogue. Nature. 1988 Aug 25;334(6184):698–700. doi: 10.1038/334698a0. [DOI] [PubMed] [Google Scholar]
  8. Frasier-Scott K., Hatzakis H., Seong D., Jones C. M., Wu K. K. Influence of natural and recombinant interleukin 2 on endothelial cell arachidonate metabolism. Induction of de novo synthesis of prostaglandin H synthase. J Clin Invest. 1988 Dec;82(6):1877–1883. doi: 10.1172/JCI113805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Furutani Y., Notake M., Yamayoshi M., Yamagishi J., Nomura H., Ohue M., Furuta R., Fukui T., Yamada M., Nakamura S. Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor. Nucleic Acids Res. 1985 Aug 26;13(16):5869–5882. doi: 10.1093/nar/13.16.5869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Förstermann U., Hertting G., Neufang B. The importance of endogenous prostaglandins other than prostacyclin, for the modulation of contractility of some rabbit blood vessels. Br J Pharmacol. 1984 Apr;81(4):623–630. doi: 10.1111/j.1476-5381.1984.tb16127.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grassi J., Frobert Y., Pradelles P., Chercuitte F., Gruaz D., Dayer J. M., Poubelle P. E. Production of monoclonal antibodies against interleukin-1 alpha and -1 beta. Development of two enzyme immunometric assays (EIA) using acetylcholinesterase and their application to biological media. J Immunol Methods. 1989 Oct 24;123(2):193–210. doi: 10.1016/0022-1759(89)90223-8. [DOI] [PubMed] [Google Scholar]
  12. Helle M., Brakenhoff J. P., De Groot E. R., Aarden L. A. Interleukin 6 is involved in interleukin 1-induced activities. Eur J Immunol. 1988 Jun;18(6):957–959. doi: 10.1002/eji.1830180619. [DOI] [PubMed] [Google Scholar]
  13. Kawakami M., Pekala P. H., Lane M. D., Cerami A. Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells. Proc Natl Acad Sci U S A. 1982 Feb;79(3):912–916. doi: 10.1073/pnas.79.3.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Martin W., Villani G. M., Jothianandan D., Furchgott R. F. Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and by methylene blue in the rabbit aorta. J Pharmacol Exp Ther. 1985 Mar;232(3):708–716. [PubMed] [Google Scholar]
  15. Mizutani H., May L. T., Sehgal P. B., Kupper T. S. Synergistic interactions of IL-1 and IL-6 in T cell activation. Mitogen but not antigen receptor-induced proliferation of a cloned T helper cell line is enhanced by exogenous IL-6. J Immunol. 1989 Aug 1;143(3):896–901. [PubMed] [Google Scholar]
  16. Nencioni L., Villa L., Tagliabue A., Antoni G., Presentini R., Perin F., Silvestri S., Boraschi D. In vivo immunostimulating activity of the 163-171 peptide of human IL-1 beta. J Immunol. 1987 Aug 1;139(3):800–804. [PubMed] [Google Scholar]
  17. Ogle J. D., Noel J. G., Balasurbramaniam A., Sramkoski R. M., Ogle C. K., Alexander J. W. Comparison of abilities of recombinant interleukin-1 alpha and -beta and noninflammatory IL-1 beta fragment 163-171 to upregulate C3b receptors (CR1) on human neutrophils and to enhance their phagocytic capacity. Inflammation. 1990 Apr;14(2):185–194. doi: 10.1007/BF00917457. [DOI] [PubMed] [Google Scholar]
  18. Okusawa S., Gelfand J. A., Ikejima T., Connolly R. J., Dinarello C. A. Interleukin 1 induces a shock-like state in rabbits. Synergism with tumor necrosis factor and the effect of cyclooxygenase inhibition. J Clin Invest. 1988 Apr;81(4):1162–1172. doi: 10.1172/JCI113431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Palmer R. M., Ferrige A. G., Moncada S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 1987 Jun 11;327(6122):524–526. doi: 10.1038/327524a0. [DOI] [PubMed] [Google Scholar]
  20. Poubelle P. E., Borgeat P., Rola-Pleszczynski M. Assessment of leukotriene B4 synthesis in human lymphocytes by using high performance liquid chromatography and radioimmunoassay methods. J Immunol. 1987 Aug 15;139(4):1273–1277. [PubMed] [Google Scholar]
  21. Poubelle P. E., Grassi J., Pradelles P., Marceau F. Pharmacological modulation of interleukin 1 production by cultured endothelial cells from human umbilical veins. Immunopharmacology. 1990 Mar-Apr;19(2):121–130. doi: 10.1016/0162-3109(90)90047-i. [DOI] [PubMed] [Google Scholar]
  22. Pradelles P., Grassi J., Maclouf J. Enzyme immunoassays of eicosanoids using acetylcholine esterase as label: an alternative to radioimmunoassay. Anal Chem. 1985 Jun;57(7):1170–1173. doi: 10.1021/ac00284a003. [DOI] [PubMed] [Google Scholar]
  23. Ritchie D. G., Fuller G. M. Hepatocyte-stimulating factor: a monocyte-derived acute-phase regulatory protein. Ann N Y Acad Sci. 1983 Jun 27;408:490–502. doi: 10.1111/j.1749-6632.1983.tb23268.x. [DOI] [PubMed] [Google Scholar]
  24. Simmet T., Hertting G. On the relation between contraction and prostaglandin release in rabbit mesenteric blood vessels. Eur J Pharmacol. 1980 Aug 8;65(4):325–331. doi: 10.1016/0014-2999(80)90335-0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES