Figure 3.

NusA stimulates and Zn²⁺ inhibits BOXB binding by Nun. (A) NusA stimulates Nun binding to ³²P-BOXB. Protein concentrations are given in nM. (B) CtP competes with Nun for NusA. Protein concentrations are given in μM. AB2, allatostatin B2. Band intensity values are given as the sum of all points over background, values are in thousands. (C) Zn²⁺ inhibits Nun binding to ³²P-BOXB. Protein concentrations are given in μM. All lanes contain ³²P-BOXB (20nM). Binding to ³²P-BOXB was assayed by gel mobility shift assays (see Materials and Methods).